In this experiment we selected six Chinese No.l miniature pigs to produce 51 pigs by laparohysterectomy-birth in the isolators, 7 pigs were shifted out because of too light body weight or dead fetus. 44 pigs existed with the surgery etistense percentage of 86.3%. After stopping lactation 36 pigs existed (81 .8%). In 10 weeks of feeding period, we checked and tested feces, feeding material, drinking-water, wind inlet /outlet and feces outlet for the anaerobic bacteria, aerobic bacteria, fungus, separately at 21d, 42d, 63d by the methods of USP, NB, SB, and all the test results were negative. Pig Pasteurella bacteria, Swine erysipelas bacteria, foot and mouth disease (FMD) virus, swine fever virus, pig mini-virus, toxoplasma were detected with all the negative results both on the mother pig before laparohysterectomy-birth and on the new-born pigs at 45d after the birth. The new-born pigs fulfill the SPF standards.