A new medium combined with the use of oblique illamination technique was developed to rapidly identify the colonies of listeria. Results were compared with those reported by foreign authors using media containing antibacterial agents,which showed methods developed in this paper had some advantages in term of rapidness and correctness for identifying listeria. Several selective-enrichment media were comparted and investigated. Results showed that CRT was the best one. Using CRT with new differentiation medium, Sen-etivity of isolating listeria increased 5-10 fold. Method of liver homogenate to isolate listeria from animals was simpified and showed very practical when used with CRT to isolate listeria from artificial infected animals. Methods of CRT and liver homogenate were used to isolate listeria from labora tory animals from some institutes in Beijing. Results were negative.