Objective To explore the effect of compound Ejiao slurry on mouse uterus. Methods Female mice were randomly divided into four groups: sham operation group, sham operation with Chinese medicine group, ovariectomized group, and ovariectomized with Chinese medicine group. The sham operation with Chinese medicine group and ovariectomized with Chinese medicine group were treated with compound Ejiao slurry gavage. HE staining, immunohistochemical staining, and Western blot were used to observe and compare the endometrium among the groups. Results Compared with that of the sham operation group, the endometrium of the sham operation with Chinese medicine group was thicker. In addition, the number of glands and the expression of vascular endothelial growth factor?A (VEGF?A) in endometrium slightly increased ( P < 0. 05). However, the differences between the ovariectomized group and ovariectomized with Chinese medicine group were not significant. Conclusions The compound Ejiao slurry can affect the growth of reproductive organs in female mice. It may regulate the endometrium by acting on the ovary.