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吴剑平,谢倩,陈林,张军峰,史丽云,詹瑧.咪喹莫特对BALB/ c 和C57BL/6 小鼠银屑病样皮损诱导作用的比较[J].中国比较医学杂志,2018,28(9):1~6.
咪喹莫特对BALB/ c 和C57BL/6 小鼠银屑病样皮损诱导作用的比较
Comparison of imiquimod?induced psoriatic lesions in BALB / c andC57BL / 6 mice
投稿时间:2018-04-12  
DOI:10.3969/j. issn. 1671 -7856. 2018. 09. 001
中文关键词:  银屑病小鼠模型  咪喹莫特  BALB/ c 小鼠  C57BL/6 小鼠  IL?23/ IL?17 轴
英文关键词:psoriasis mouse model  imiquimod  BALB/ c mice  C57BL/6 mice  IL?23/ IL?17 Axis
基金项目:国家自然科学基金项目(编号:81473593,81473458)
作者单位E-mail
吴剑平 南京中医药大学,南京 210023 wjp4411544@163. com 
谢倩 南京中医药大学,南京 210023  
陈林 南京中医药大学,南京 210023  
张军峰 南京中医药大学,南京 210023  
史丽云 南京中医药大学,南京 210023  
詹瑧 南京中医药大学,南京 210023 zhanzhan5607@163. com 
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中文摘要:
      目的 观察咪喹莫特对不同品系小鼠银屑病样皮损诱导作用的差异及其作用机理?方法 选用BALB/ c 和C57BL/6 两种小鼠品系,分别在其背部涂抹62. 5 mg 含5% 咪喹莫特的明欣利迪乳膏,在涂抹2,4,6 d后,采用PAIS 评分观察小鼠背部皮肤形态学变化;HE 染色观察涂药局部皮肤病理组织学变化,显微镜下测量表皮厚度;实时荧光定量PCR 法测定IL?23/ IL?17 轴相关细胞因子的相对表达量?结果 随咪喹莫特涂抹天数的增加, BALB/ c 小鼠和C57BL/6 小鼠背部皮肤均逐渐出现鳞屑?红斑?表皮增厚等银屑病样表现?与同时间段C57BL/6小鼠银屑病样皮损比较,BALB/ c 小鼠皮损PAIS 评分更高( P < 0. 05 或 P < 0. 01),病理组织学改变及表皮增厚均更显著( P < 0. 05 或 P < 0. 01)?涂抹咪喹莫特2 d 后,两种小鼠IL?23?IL?17A 和IL?17F mRNA 相对表达量均显著升高,且均呈现先高后低的动态变化?其中BALB/ c 小鼠表达峰值出现于涂药后2 d,C57BL/6 小鼠表达峰值出现于涂药后4 d?结论 咪喹莫特均可诱导BALB/ c 和C57BL/6 小鼠出现人类银屑病样皮损,其中BALB/ c 小鼠银屑病样皮损更典型,可能更适用于人类银屑病研究,IL?23/ IL?17 轴的激活是皮损出现的重要诱导因素之一?
英文摘要:
      Objective To determine the effect of imiquimod on psoriasis?like lesions in different strains of mice and the mechanism involved. Methods Two mouse strains, BALB/ c and C57BL/6, were chosen, onto the back of which 62. 5 mg of 5% imiquimod cream was applied. PAIS score was used to determine the morphological changes of mouse back skin. The histopathological changes of skin were observed by HE staining, the thickness of the epidermis was measured under a microscope, and the relative expression of cytokines related to the IL?23/ IL?17Axis was measured by real?time PCR. Results With the passage of time, psoriasis?like skin lesions gradually formed on the back skin of both BALB/ c and C57BL/6 mice. Compared with those in C57BL/6 mice, the PAIS score of BALB/ c mice was higher, and histopathological changes and epidermal thickening ( P < 0. 05 or P < 0. 01) were also more significant. Two days after the application of imiquimod, the relative expression of IL?23, IL?17A, and IL?17F mRNA in the two mouse groups all increased significantly, and both groups showed a dynamic change of an initial increase and then a decrease. The peak of expression in BALB/ c mice appeared 2 days after the application, and in C57BL/6 mice it appeared at 4 days. Conclusions Imiquimod can induce psoriasis?like lesions in both BALB/ c mice and C57BL/6 mice, but the lesions in BALB/ c mice appeared to be more typical, potentially making them more suitable for human psoriasis research. The activation of the IL?23/ IL?17Axis is one of the most important inducers of skin lesions.
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