Improvement and evaluation of experimental animal models of rats with coronary atherosclerosis
Received:July 03, 2013  Revised:July 23, 2013
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KeyWord:Wistar rat; Atherosclerosis; coronary heart disease;D3vitamin; Hypercholesterolemia;
李同达 中国中医科学院
李冬梅 中国中医科学院
徐慧 中国中医科学院研究生院
宗文静 中国中医科学院研究生院
曹洪欣 国家中医药管理局、中国中医科学院
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      This research builds the improved method of atherosclerosis rats mold based on the literature, which aims to establish a suitable coronary heart disease wistar rats model for cardiovascular research. To overcome the wistar rats’s resistance to cholesterol induce atherosclerosis, animals were given D3 vitamin at 150000 IU :rat per month associated with cholesterol-enriched diet for first 3 months, following 2 months of high cholesterol diet alone. Blood serum parameters (at days 0,30,90,120 and 150) and coronary artery, aortic and heart morphology (at days 120 and 150)were examined. Other animals receiving a normal diet were used as a control group. Results showed that at day 90 severe hypercholesterolemia, elevated blood serum LDL-C and CHO, oxidized LDL, AngⅡ,sICAM-1.Lesions were characterized by widening of the first interlamellar spaces in the aorta, fibrosis of coronary arterial arterial wall and recent foci of myocardial fibrosis. At day 150d oxidized LDL, AngⅡ,sICAM-1 and ET-1were more enhanced. The D3 vitamin administration induced advanced atherosclerotic lesions in arterial wall, represented by the rupture of elastic lamellae, smooth muscle cell proliferation and lipid-calcic core. The complicated plaque frequently evolved into ulcerations. The ischaemic effects were represented by acute myocardial infarction. D3 vitamin is an atherogenic agent which, when associated with hypercholesterolemia, allows the development of advanced atherosclerotic lesions in wistar rat which resembles human plaque.
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