Murine Hepatitis Virus（MHV）is a corona RNA virus, usually subclinical infection. The quality of the experimental animals and the results of animal experiments are affected. The epidemic scope can be controlled and the impact on the results of animal experiments can be prevented by timely detection of MHV. This article reviews MHV detection and diagnosis methods in recent years, analyzed MHV infection in experimental animals and animal experiments, and analyzed the pros and cons of those detection methods to provide a theoretical basis for further research.