一种啮齿类动物脑内微透析记录的清醒活动装置的设计和应用研究

张钰; 周澧; 张玲; 孙秀萍; 李祥磊; 秦川

Design and application of a rodent awake activity device for brain microdialysis recording

Received:February 23, 2022

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DOI：10. 3969 / j.issn.1005-4847. 2022. 03. 007

KeyWord:neurotransmitter; microdialysis; awake activity device; dopamine; striatum; substantia nigra

Author	Institution
张钰	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京
周澧	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京
张玲	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京
孙秀萍	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京
李祥磊	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京
秦川	1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 ;2. 国家人类疾病动物模型资源库,北京 ;3. 国家卫生健康委员会人类疾病比较医学重点实验室,北京 ;4. 北京市人类重大疾病实验动物模型工程技术研究中心,北京

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Abstract:

Objective To design and construct a rodent awake activity device for brain microdialysis recording and achieve the application and verification of brain microdialysis sampling in mice. Methods The rodent awake activity device for brain microdialysis recording was designed and constructed using relevant materials. Dopamine-related neurotransmitters in the substantia nigra-striatum neural circuit in mice were measured and verified by this device. Microdialysis probes were implanted into the substantia nigra and striatum. Recording and microdialysis sampling were performed for 7 days after operation. Dopamine and its metabolic intermediate DOPAC were analyzed in the substantia nigra and striatum. The microdialysis flow rate was 2 μL/ minute. Samples were collected every 20 minutes for 200 minutes to analyze DA and DOPAC concentration changes over time. Results ( 1) Mouse brain samples showed stable levels of neurotransmitters. (2)Dopamine concentrations were (0. 11 ± 0. 01) nmol / L in the substantia nigra and (0. 22 ± 0. 05) nmol / L in the striatum. DOPAC concentrations were (3. 38 ± 0. 59) nmol / L in the substantia nigra and (20. 35 ± 6. 87) nmol / L in the striatum. No significant differences were found between each sampling period. Conclusions The rodent awake activity device for brain microdialysis recording is practical and stable with good applications in time and space dimensions. Two national patents have been applied for and authorized: Patent for invention ( 202110525342. 5) and Patent for utility models (202121027247. 4).