Objective To obtain a novel coronavirus pneumonia transgenic mouse model with constitutive human angiotensin converting enzyme 2 (hACE2) gene expression, that can efficiently maintain human physiological levels of a bacterial artificial chromosome ( BAC) hACE2 gene. This model could provide an important tool for research into cardiovascular disease, the pathogenesis of novel coronavirus pneumonia, and the development of drugs and vaccines. Methods A BAC plasmid containing the complete coding sequence of human ACE2 (hACE2) was purified and transgenic mice were established by fertilized egg microinjection and Fallopian tube transplantation. Stable F2 mouse strains were obtained by cross-breeding and analyzed in relation to integration of the hACE2 gene, gene copy number, relative fluorescence quantitative polymerase chain reaction (PCR), Western Blot, and immunofluorescence. Results Four mouse strains (hACE2-6-9, hACE2-14-3, hACE2-15-1 and hACE2-15-2) were obtained. The result of gene integration showed that the hACE2-6-9, hACE2-15-1 and hACE2-15-2 strains contained the complete hACE2 gene locus and long gene regulatory region, while hACE2-14-3 contained the complete hACE2 gene locus and a short gene regulatory 3' untranslated region. Relative fluorescence quantitative PCR and western blot showed that hACE2 mRNA and protein levels in the intestine were lower in the hACE2-6-9, hACE2-15-1 and hACE2-15-2 strains, while expression levels were higher in the hACE2-14-3 strain with shorter regulatory sequences. Immunofluorescence staining showed that hACE2 was expressed in renal tubular epithelial cells and pulmonary vascular endothelial cells in hACE2-15-1 mice. Conclusions We obtained novel coronavirus pneumonia hACE2 transgenic mice with the full-length gene sequence and the complete promoter and gene regulatory regions. This model will provide an important tool for the study of cardiovascular diseases and the pathogenesis of novel coronavirus pneumonia, as well as for examining mechanisms regulating hACE2 gene expression and the development of drugs and vaccines.