Preliminary establishment of chronic thrombus model in mice
Received:June 16, 2020  
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DOI:10. 3969 / j.issn.1005-4847. 2021. 01. 011
KeyWord:chronic thrombosis; mice; carrageenan; oxidative stress; inflammatory cytokines
                    
AuthorInstitution
韩子逸 扬州大学兽医学院,江苏 扬州
沈诗怡 扬州大学兽医学院,江苏 扬州
张金鑫 扬州大学兽医学院,江苏 扬州
周燕 扬州大学兽医学院,江苏 扬州
赵昕 扬州大学兽医学院,江苏 扬州
刘明江 1.扬州大学兽医学院,江苏 扬州 ; 2.江苏省动物重要疫病与人畜共患病防控协同创新中心, 江苏 扬州
徐霄龙 首都医科大学附属北京中医医院,北京
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Abstract:
       Objective To screen the conditions of a chronic thrombus mouse model after injecting a carrageenan solution at various concentrations intraperitoneally. Methods Seventy ICR male mice were randomly divided into seven groups with 10 mice in each group: blank ( control), 0. 02%, 0. 04%, 0. 06%, 0. 08%, 0. 10%, and 0. 20% groups. Mice in the blank group were intraperitoneally injected with normal saline (0. 01 mL/ g), whereas mice in the other groups were intraperitoneally injected with carrageenan solutions at the corresponding concentration (0. 01 mL/ g) once a day. Mice in each group kept at (20 ± 1)°C and had free access to food and water. The black-tailed conditions (black-tailed rate and length) of each group were observed each day. An outlet plug was used for sampling. Blood samples were collected to detect blood clots, and indicators of oxidative stress and inflammatory factors in colon tissues and plasma were detected. Results In the 0. 06% dose group, two mice were thrombotic on 5 d and eight mice were thrombotic on day 6. Compared with the blank group, FIB content in the 0. 06% dose group was increased significantly ( P< 0.05), APTT was shortened significantly (P< 0.05), TT was prolonged significantly (P< 0.05), and PT was prolonged significantly (P< 0.01). SOD and GSH-Px in colon tissue and plasma of the 0. 06% dose group were decreased significantly (P< 0.01), and MDA was increased significantly (P< 0. 05). The colon tissue and plasma levels of TNF-α and IL-1β in the 0. 06% dose group were increased significantly (P< 0.05), and those of IL-10 were decreased significantly (P< 0.05). Conclusions A 0. 06% carrageenan solution (0.01 mL/ g) injected intraperitoneally for 6 d ( once a day) at (20 ± 1)°C is the optimal condition to establish a mouse model of chronic thrombosis.
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