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| Standard and method of preparation and verification of Clostridium perfringens (C) for production and testing of veterinary vaccines |
| Received:June 01, 2019 |
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| DOI：10. 3969 / j.issn.1005-4847. 2020. 05. 006 |
| KeyWord:Clostridium perfringens; CVCC60101; preparation; verification; standard Conflicts of Interest: The authors declare no conflict of interest. |
| Author | Institution |
| 彭国瑞 |
1.中国兽医药品监察所,北京 ; 2.中国农业大学动物医学院,北京 |
| 李旭妮 |
中国兽医药品监察所,北京 |
| 王秀丽 |
中国兽医药品监察所,北京 |
| 李伟杰 |
中国兽医药品监察所,北京 |
| 魏津 |
中国兽医药品监察所,北京 |
| 马欣 |
中国兽医药品监察所,北京 |
| 张一帜 |
中国兽医药品监察所,北京 |
| 田野 |
中国兽医药品监察所,北京 |
| 刘博 |
中国兽医药品监察所,北京 |
| 李建 |
中国兽医药品监察所,北京 |
| 罗玉峰 |
中国兽医药品监察所,北京 |
| 李俊平 |
中国兽医药品监察所,北京 |
| 彭小兵 |
中国兽医药品监察所,北京 |
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| Abstract: |
| Objective To prepare Clostridium perfringens (CVCC60101) for production and testing of veterinary vaccines, verify its biological characteristics, and perfect the standard and method of preparation and verification. Methods In this study, Clostridium perfringens (C) prepared and stored by freeze-drying in 1960 was resuscitated, rejuvenated using susceptible animals, isolated, cultured, freeze-dried, and the new strain’s morphology, culture characteristics, biochemical properties, serological characteristics, virulence, immunogenicity, and other characteristics were tested. Results The result showed that the new strain was pure, and its morphology, culture characteristics, and biochemical properties accorded with the characteristics of Clostridium perfringens. In addition, its vacuum degree, residual moisture, serological characteristics, virulence, and immunogenicity accorded with the standard of the strain for production and testing of veterinary vaccines. Conclusions This research gives more experimental data for perfecting the standard and method of preparation and verification of Clostridium perfringens (C). |
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