Exploration of the mechanism of Taizishen Zhike Yiqi Powder in the treatment of asthma in rats
Received:April 17, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 017
KeyWord:Taizishen Zhike Yiqi powder; asthma; T helper cell; cytokine; oxidative stress; rats
赵晨 华中科技大学同济医学院附属协和医院儿科,武汉 
赵娇 华中科技大学同济医学院附属协和医院儿科,武汉 
赵晓波 华中科技大学同济医学院附属协和医院儿科,武汉 
白燕 华中科技大学同济医学院附属协和医院儿科,武汉 
金润铭 华中科技大学同济医学院附属协和医院儿科,武汉 
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      Objective To explore the effect and mechanism of formula TaizishenZhikeYiqi Powder (TZY) onimproving the symptoms of bronchial asthma rats. Methods Rats were randomly divided into 7 groups: control group,asthma model (OVA inducted), low dose group of traditional Chinese medicine, medium dose group of traditional Chinesemedicine, high dose group of traditional Chinese medicine, Pulmicort respules group, group of Pulmicort respules andtraditional Chinese medicine. HE staining was used to detect the submucocal inflammatory cell infiltration in the bronchialmucosa. Flow cytometry was used to detect the ratio of Th1 to Th2 cells in each group. The levels of IL-4, IL-5, IgE andIFN-γ in bronchoalveolar lavage fluid (BALF) of each group were detected by ELISA. The levels of SOD, MDA, GSH andT-AOC in BALF of each group were detected by biochemistry. Results TZY reduced airway inflammation exhibiting atherapeutic effect on asthma in the rats. Compared with the asthma group, the ratio of Th1 to Th2 cells in the TZY groupand joint use group increased significantly, the levels of IL-4, IL-5 and IgE decreased significantly, but the level of IFN-γincreased significantly ( P <0. 05) in a dose-dependent manner. Compared with the asthma group, the levels of GSH, T中AOC and SOD in the TZY groups and joint drug use group were significantly increased, and the level of MDA wassignificantly decreased. The TZY and Pulmicort respules combination group exerted similar or better effects than thePulmicort respules atomization. Conclusions TZY can alleviate the inflammatory state of bronchial mucosa in rats. Themechanism may be related to up-regulating the ratio of Th1 to Th2 cells, regulating inflammatory factors and oxidativestress.
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