Effects of chronic PM2. 5 exposure on lung inflammation and NLRP3 inflammasome activation in C57BL / 6J mice
Received:February 21, 2019  
View Full Text  View/Add Comment  Download reader
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 004
KeyWord:mouse; PM2. 5; NLRP3 inflammasome; lung inflammation
丁世彬 1. 新乡医学院,河南新乡 ; 2. 河南省分子诊断与检验医学协同创新中心,新乡 
高丽云 新乡医学院,河南新乡 
李玉春 新乡医学院,河南新乡 
卜勇军 新乡医学院,河南新乡 
张国富 新乡医学院,河南新乡 
Hits: 1576
Download times: 562
      Objective  To study the effects of chronic PM2. 5 exposure on lung inflammation and NLRP3inflammasome activation in mice, and to provide a new target for prevention and treatment of lung injury caused by PM2. 5.Methods Male C57BL/6J mice were exposed to two doses of PM2. 5 by tracheal instillation [2, 10 mg/ (kg.bw)], andthe control mice were instilled with normal saline. After mice had been instilled for 20 times (1 time every 3 days), bloodand lung tissues were collected. Blood cells were counted, and lung tissue macrophage levels were measured usingimmunofluorescence staining. Interleukin (IL)-1β and IL-18 levels and caspase-1 activity in lung tissues were determinedusing ELISA and caspase-1 activity measurement kits. The expression levels of NLRP3 inflammasome-associated mRNA inlung tissue were detected using real-time PCR. Results The two doses of PM2. 5 significantly reduced the percentage ofmonocytes ( P < 0. 01) and induced lung inflammation. The PM2. 5-treated mice had a higher percentage of neutrophils ( P < 0. 01), a higher in caspase-1 activity ( P < 0. 01), and a higher in mRNA expression of NLRP3 and ASC ( P < 0. 01) inlung tissues compared with the control mice. IL-1β and IL-18 levels in the lung tissues of the two PM2. 5-exposed groupswere significantly higher than those seen in the control group (both P < 0. 01). Conclusion Chronic PM2. 5 exposure may induce lung inflammation by activating the NLRP3 inflammasome in the lung tissue.
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=970261AF74811A5D5BA3361057D5C78565ED048F86CE5149A5C9C0A17EF62BFAAB7435FDDA25BE870DB3CAE6AC02675BD7604BBED7CF48E8DE8E06F2A41877A7A1AADEF561F335304EB29364198937BB8839B0765C3BE51848A28268C92072C42DA2D610423C411D3F0625866668637B&pcid=&cid=&jid=&yid=B6351343F4791CA3&aid=&vid=&iid=E158A972A605785F&sid=68FDAD96FCC0AB1B&eid=78BF76CF5B7CB0F2&fileno=201904004&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="B6351343F4791CA3"; var my_aid="";