The role of PKCzeta-expression in the anterior cingulate cortex in emotional response to complete Freund’s adjuvant-induced inflammatory pain
Received:December 15, 2015  
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DOI:10.3969/j.issn.1005-4847.2016.04.015
KeyWord:Inflammatory pain;Emotion;Anterior cingulate cortex;Complete Freund's adjuvant;PKCzeta;Rat
                 
AuthorInstitution
杜俊英 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
温存 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
邵晓梅 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
房军帆 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
梁宜 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
方剑乔 浙江中医药大学 第三临床医学院 针灸神经生物学实验室, 杭州
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Abstract:
      Objective To observe the emotional response to inflammatory pain model induced by complete Freund’s adjuvant (CFA), and to explore the relationship between phosphate kinase C (PKC)zeta in the anterior cingulate cortex (ACC) and emotional response to inflammatory pain. Methods Twenty-four healthy 2-month old female Sprague-Dawley rats were randomly divided into control group and model group. The inflammatory pain model was established by injecting subcutaneously with 0.1 ml CFA into the right hind paw of rats. To observe the dynamical changes of body weight and pain threshold before modeling, and at 3, 7, 14, 21, and 28 days after modeling. Detecting the total distance, distance in open arm, the number of times entering into the open arm, percentage of time spent in open arm of elevated O maze were measured at 14, 21, 28 days after modeling. The total distance, distance in the central quadrat, the number of times entering the central quadrat, and time spent in the central quadrat of open field were determined at 14 and 29 days after modeling. The protein expression of PKCzeta in the ACC was detected by Western blot at 14 and 29 days after CFA-injection.Results There were no significant differences of body weight between the control and model groups (P>0.05). Compared with the control group, the pain threshold of rats in the model group was significantly decreased at 1, 3, 7, 14, 21, and 28 days after CFA-injection (P<0.05), the distance in open arm and percentage of time spent in open arm of the model group were markedly reduced at 28 days after modeling (P<0.05), distance in the central quadrat and the number of times entering into the central quadrat of model group were significantly decreased at 29 days after modeling (P<0.05), and the protein expression of PKCzeta in ipsilateral ACC was increased at 29 days after CFA-injection (P<0.05). There was a negative correlation between distance in open arm and protein expression of PKCzeta in the ipsilateral ACC, percentage of time spent in open arm and protein expression of PKCzeta in the ipsilateral ACC, distance in the central quadrat and protein expression of PKCzeta in ipsilateral ACC, the number of times entering into the central quadrant and protein expression of PKCzeta in the ipsilateral ACC, respectively. Conclusions Abnormal emotional behavior may occur in rats with chronic inflammation pain induced by CFA-injection. This pain emotion behavior may be associated with high protein expression of PKCzeta in the ipsilateral anterior cingulate cortex.
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