Establishment of an orthotopic implantation model of hepatocellular carcinoma in nude mice
Received:November 23, 2015  
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KeyWord:BALB/c nude mice;Liver cancer;Orthotopic implantation;Small animals living imaging system
田树红 海南医学院 海南省药物安全性评价研究中心, 海口
王日超 海南医学院 海南省药物安全性评价研究中心, 海口
邢桂兰 海南医学院 海南省药物安全性评价研究中心, 海口
肖敏 海南医学院 海南省药物安全性评价研究中心, 海口
符健 海南医学院 海南省药物安全性评价研究中心, 海口
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      Objective To establish a new orthotopic implantation model of hepatocellular carcinoma in BALB/c nude mice. Methods The nude mice were under general anesthesia with isoflurane inhalation. Hepatocellular carcinoma was induced by subcutaneous inoculation of human hepatocellular carcinoma Bel-7402 cells in nude mice. The orthotopic implantation model of hepatocellular carcinoma in nude mice was established by orthotopic implantation of small pieces of the liver cancer tissues into the mouse liver by either a sandwich method or embedding method. The post-operative tumor growth was determined by a small animal in vivo imaging system. Histopathological examination of the liver and tumor tissues was performed at four weeks after modelling. Results The orthotopic hepatocellular carcinoma model of nude mice established by the sandwich method had several distinct advantages:shorter operative time, less operational difficulties, with a high rate of tumor formation, no wide-spread peritoneal tumor dissemination, well-preserved tumor histological structure, and low animal mortality. Conclusions Compared with the embedding method, the sandwich method can save much time for preparation of this orthotopic implantation model of hepatocellular carcinoma in nude mice, and provide a more convenient platform for research of liver cancer pharmacodynamics and other biological studies.
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