Objective To study the effect of low frequency electrical stimulation on blood hormone levels in different animals. Methods At 12 h, 24 h and 36 h after low frequency electrical stimulation to Chinese tree shrews, Wistar rats, and BALB/c mice, respectively, the blood noradrenaline (NA) and endothelin (ET) levels were determined by radioimmunoassay (RIA). Results The blood NA and ET levels of electrical stimulation group were significantly higher than that in the control group (P < 0.01). At 24 h after reserpine treatment, the same electrical stimulation was given. The NA, ET levels were significantly decreased compared with that in the electrical stimulation group (P< 0.05 to 0.01). Different animals responded to the same electric stimulus differently, following the order of Chinese tree shrews > Wistar rats > BALB/c mice. Conclusions Low frequency electrical stimulation can promote the release of noradrenaline and endothelin in Chinese tree shrews, Wistar rats and BALB/c mice. Reserpine can reduce the stress induced by low frequency electric stimulation to the body.