Bone marrow mesenchymal stem cells inhibit inflammatory response of microglial cells
Received:December 11, 2013  
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KeyWord:Bone marrow mesenchymal stem cells;Microglia;Inflammatory response
张凯华 首都医科大学, 北京市神经外科研究所, 北京
王飞 首都医科大学, 北京三博脑科医院, 北京
胡慧敏 首都医科大学, 北京市神经外科研究所, 北京
黄华 首都医科大学, 北京市神经外科研究所, 北京
安沂华 首都医科大学, 北京三博脑科医院, 北京 ;武警总医院细胞移植科, 北京
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      Objective To investigate the inhibitory effect of bone marrow mesenchymal stem cells (BMMSC) on the inflammatory response of microglial cells. Methods The samples were divided into four groups. Group Ⅰ: microglial (BV-2) cells were cultured in DMEM (high glucose). Group Ⅱ: BV-2 cells were cultured in DMEM containing lipopolysaccharide (LPS). Group Ⅲ: BV-2cells and BMMSCs were co-cultured in DMEM. Group Ⅳ: BMMSCs were cultured in DMEM containing LPS. The growth state and ultrastructure of BV-2 cells were observed and the changes of TNF-α expression were detected. Results Different cell densities of BV-2 cells were observed under the optical microscope in an order from high level to low level: group Ⅰ > group Ⅲ > group Ⅱ. The expressions of TNF-α were: group Ⅱ > group Ⅲ > group Ⅰ. Ultrastructural observation of BV-2 cells showed that there were a large number of swollen mitochondria and endoplasmic reticulum, some of them showed vacuolization. No BV-2 cells with multiple hucleoli were found in the group Ⅱ indicating the absence of active cell growth. At the same time, cytolysis was observed only in the group Ⅱ. The growth of BV-2 cells in the group Ⅲ was better than that in the group Ⅱ.Conclusions BMMSCs can inhibit inflammatory response of microglial cells, therefore, play a neuroprotective role.
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