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| Establishment of a rat model simulating the high altitude pulmonary edema |
| Received:August 29, 2013 |
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| DOI：10.3969/j.issn.1005-4847.2014.01.015 |
| KeyWord:High altitude pulmonary edema;Rats;hypobaric chamber;TNF-α;IL-6 |
| Author | Institution |
| 陶义存 |
新疆医科大学基础医学院, 乌鲁木齐 |
| 是文辉 |
兰州军区乌鲁木齐总医院, 乌鲁木齐 |
| 许永华 |
兰州军区乌鲁木齐总医院, 乌鲁木齐 |
| 靳春丽 |
新疆医科大学基础医学院, 乌鲁木齐 |
| 卢伟 |
新疆医科大学基础医学院, 乌鲁木齐 |
| 李琳琳 |
新疆医科大学基础医学院, 乌鲁木齐 |
| 毛新民 |
新疆医科大学基础医学院, 乌鲁木齐 |
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| Abstract: |
| Objective To establish a rat model simulating high-altitude pulmonary edema by using hypobaric chamber. Methods Sixty healthy Wistar rats (male:female=1:1) were randomly divided into five groups: control group, hypoxia 24 h group, hypoxia 48 h group, hypoxia 72 h group, and hypoxia 7 d group. Except for the control group, hypoxia group rats were put in hypobaric chamber for the given time. The lung water content, contents of IL-6 and TNF-α in the lung homogenates were assessed and histological examination was conducted on all animals. Results Compared with the control group, the lung water content, content of IL-6 and TNF-α in the lung homogenates were significantly increased in the hypoxia 24 h group, hypoxia 48 h group and hypoxia 72 h group, while lung water content, content of IL-6 and TNF-α in the lung homogenates were lower than that in the hypoxia 72 h group. Interstitial pulmonary edema and inflammatory cell infiltration, alveolar septal widening and interstitial hyperemia were found in the hypoxia 72 h group. Conclusion A rat model of high altitude pulmonary edema is successful set up by putting the rats in hypobaric chamber for 72 h. |
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