Establishment of a rat model simulating the high altitude pulmonary edema
Received:August 29, 2013  
View Full Text  View/Add Comment  Download reader
DOI:10.3969/j.issn.1005-4847.2014.01.015
KeyWord:High altitude pulmonary edema;Rats;hypobaric chamber;TNF-α;IL-6
                    
AuthorInstitution
陶义存 新疆医科大学基础医学院, 乌鲁木齐
是文辉 兰州军区乌鲁木齐总医院, 乌鲁木齐
许永华 兰州军区乌鲁木齐总医院, 乌鲁木齐
靳春丽 新疆医科大学基础医学院, 乌鲁木齐
卢伟 新疆医科大学基础医学院, 乌鲁木齐
李琳琳 新疆医科大学基础医学院, 乌鲁木齐
毛新民 新疆医科大学基础医学院, 乌鲁木齐
Hits: 1440
Download times: 1219
Abstract:
      Objective To establish a rat model simulating high-altitude pulmonary edema by using hypobaric chamber. Methods Sixty healthy Wistar rats (male:female=1:1) were randomly divided into five groups: control group, hypoxia 24 h group, hypoxia 48 h group, hypoxia 72 h group, and hypoxia 7 d group. Except for the control group, hypoxia group rats were put in hypobaric chamber for the given time. The lung water content, contents of IL-6 and TNF-α in the lung homogenates were assessed and histological examination was conducted on all animals. Results Compared with the control group, the lung water content, content of IL-6 and TNF-α in the lung homogenates were significantly increased in the hypoxia 24 h group, hypoxia 48 h group and hypoxia 72 h group, while lung water content, content of IL-6 and TNF-α in the lung homogenates were lower than that in the hypoxia 72 h group. Interstitial pulmonary edema and inflammatory cell infiltration, alveolar septal widening and interstitial hyperemia were found in the hypoxia 72 h group. Conclusion A rat model of high altitude pulmonary edema is successful set up by putting the rats in hypobaric chamber for 72 h.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=909BCF45EAE28FE889FFC352FC5C360F8DBC1DB95F90B331055667B92A50CCAFC5C492E2769A8A86F487996200AF09B3B7A655426EDCE5509F5C83D4DEB9C915DCABEB341280F0F62D8FC99E4A603260&pcid=&cid=&jid=&yid=9EAD63ADE6B277ED&aid=&vid=&iid=CA4FD0336C81A37A&sid=228A710F49B6CE58&eid=46CB27789995047D&fileno=20140116&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9EAD63ADE6B277ED"; var my_aid="";