PCR amplification of resistin gene from adipose tissue of ICR mice
  
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KeyWord:ICR mice;Adipose tissue;Resistin gene;Amplification;
LU Shuai-yao,ZHAO Yuan,TANG Dong-hong,HE Zhan-long,YU Wen-hai,SHAO Cong-wen
LU Shuai-yao,ZHAO Yuan,TANG Dong-hong,HE Zhan-long,YU Wen-hai,SHAO Cong-wen(Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,Kunming 650118,China)
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Abstract:
      Objective To amplify the resistin gene from adipose tissue of ICR mice for further research of its functions.Methods Total RNA was extracted from the adipose tissue for amplification of resistin gene by RT-PCR.The sequence of amplified nucleotide acids was analyzed by DNAMAN software after gel electrophoresis and sequencing.Results The total RNA had good integrity.The expected 363 bp fragment was obtained by RT-PCR.The base peak was uniform in both directions of sequencing.The amplified fragment showed a 93...
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