Objective To investigate the metabolic derivatives of the glyceryl trinitrate (GTN). Methods Rabbits were used in this work. Blood pressures were measured by dynamic analyzer, methemoglobin (MetHb) by blood gas analyzer and alanine transferase (ALT) by auto-biochemist analyzer. Nitrate, nitrite and reduced glutathione (GSH) were measured with colorimetry. Results Blood pressures and GSHs in red blood cells showed significant decrease, nitrite showed significant increase, MetHb and ALT showed no remarkable difference before and after GTN administration. Conclusion Metabolic GTN exhausts thiols and yields nitrite, and it has no effects on hemoglobin and liver.