ObjectiveThe follicular fluid (oFF) and hypoxanthine were added in maturation medium to prevent the GVBD occur,prolong the transcription of the oocyte genome, improve the maturation level of oocyte cytoplasm, enhance the IVF embryo developmental potential. Method The oocytes recovered from the ovaries collected in abattoir were cultured in vitro maturation medium containing follicular fluid(oFF) and hypoxanthine in different concentration. The nuclear phases were checked after 24hrs. Results Oocytes were incubated in culture medium with 50% and 100% follicular fluid for 24h, and 19% and 33 .3% of them were maintained in GV stage, respectively. At a concentration of 4mM hypoxanthine in culture medium, the meiosis resumption of ovine oocytes was inhibited significantly. 21 .6% of oocytes were maintained in GV stage, and 44 .6% were in PI stage. But the inhibitory effect could not be reversed by the induction of FSH. Conclusion The concentration of inhibitory factors had liner relationship with meiosis arrest. There were dose -dependent effects in the control of meiosis.