Objective To study the different usage of BALB/c and C 57 BL/6,the two strains of laboratory mouse with different genetic backgrounds,in relevant experiments Methods Authors chose antisense oligo-nucleotides of two genes expressed in central nervous system by random sequencing and bioinformatics analysis, injected them into the lateral ventricles of mouse brain using Hamilton micro-syringe respectively There are also two control groups of saline and scramble Each group has ten mice And then several behavioral tests were carried out They are Feeding for metabolic ability,Open Field Behavior for locomotion activity,Tail Flick Latency(TFL)for limen of pain,Step-down Test for memory Results It was found that after the injection of No 1 antisense both two strains of mice showed damaged memory and there were obvious differences between experimental groups and control groups It is said that the function of No 1 gene is correlative with memory After the No 2 antisense was injected,BALB/c strain experimental group showed obvious decrease in numbers of step-across and standing-up in open field behavior test compared with control groups But in C 57 BL/6 mice,there weren't obvious differences between experimental group and control groups And there were also differences between the control groups of the two strains Conclusion The results show that using laboratory mouse strains with different genetic backgrounds can set up a further monitoring system for the screening of novel gene funtion Simultaneously the results have certain significance in finding which kinds of strains are suitable for which kinds of experiments