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Clonig of Human CD55 Gene and Recombing Its Transgenic Constructs |
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DOI: |
KeyWord:CD55 gene Cloning Transgenic constructs |
Chen-Ying,Hiang Hui-min,Liu Jin-fen,Ding Wen-xiang,Su Zhao-kang,Wang Jian-rong |
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Abstract: |
First,we use human hepatic RNA as templ ate and got CD55
cDNA by PT-PCR. Secondly, CD55 cDNA is combined with promoter a nd polyA of human α-globin, then inserted into
plasmid pGEM-5zf. At last,we got a transgenic constructs' of pGEM-5zf-α-globin-CD55 cDNA'. The constructs
ma y use to found human CD55 transgenic animal model. |
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