| 孙路,付函君,刘云鹏,武婧,林凯丽,石桂英,王敬竹,孙井江,高虹.转基因人源化 ACE2 裸小鼠模型的建立[J].中国实验动物学报,2022,30(3):408~415. |
| 转基因人源化 ACE2 裸小鼠模型的建立 |
| Establishment of transgenic humanized ACE2 nude mouse model |
| 投稿时间：2021-11-26 |
| DOI：10. 3969 / j.issn.1005-4847. 2022. 03. 014 |
| 中文关键词: 宿主血管紧张素转换酶 2 转基因 人源化 裸小鼠 |
| 英文关键词:angiotensin-converting enzyme 2 transgenic humanization nude mouse |
| 基金项目: |
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| Author Name | Affiliation | E-mail | | SUN Lu | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | Suluuuuu@ outlook. com | | FU Hanjun | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | LIU Yunpeng | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | WU Jing | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | LIN Kaili | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | SHI Guiying | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | WANG Jingzhu | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | SUN Jingjiang | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | | | GAO Hong | Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China | gaohongdws@ aliyun. com |
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| 中文摘要: |
| 目的 建立 BALB/ c-Nude 裸小鼠为背景的人源化 ACE2( hACE2) 转基因裸小鼠动物模型。 方法 利用 hACE2 转基因小鼠与雄性 BALB/ c-Nude 裸小鼠杂交获得 F1 代,将 F1 代 hACE2 小鼠与 BALB/ c-Nude 裸小鼠回交获得 F2 代,再将 F2 代 hACE2 小鼠互交获得 F3 代 hACE2 转基因裸小鼠。 对 F3 代中 hACE2 转基因裸小鼠的生长发育、生理指标及免疫指标与 C57BL/ 6J 野生型小鼠、hACE2 小鼠和 BALB/ c-Nude 裸小鼠对比分析。 结果 (1)hACE2 转基因裸小鼠生长发育指标与 C57BL/ 6J 野生型小鼠、hACE2 小鼠和 BALB/ c-Nude 裸小鼠无明显差异。 (2)hACE2 转基因裸小鼠生理指标中,建立的 hACE2 转基因裸小鼠与 BALB/ c-Nude 裸小鼠相似,病理解剖观察发现,小鼠体内均无胸腺。 脏器系数结果与 BALB/ c-Nude 裸小鼠比较,脾系数和肝系数出现显著性差异(P< 0. 05)。 血常规检测指标与 C57BL/ 6J 野生型小鼠、hACE2 小鼠比较,中性粒细胞百分比(NEU)、淋巴细胞百分比(LYM)和 单核细胞百分比(MONO)均出现显著性差异(P< 0. 01)。 (3)hACE2 转基因裸小鼠免疫指标中,与 C57BL/ 6J 野生型小鼠和 hACE2 小鼠比较,CD45+CD3+、CD45+CD3+CD4+、CD45+CD11B+和 CD45+NK+均出现显著差异(P< 0. 01),与 BALB/ c-Nude 裸小鼠无明显差异。 结论 成功建立了 BALB/ c-Nude 为背景的 hACE2 转基因免疫缺陷裸小鼠动物模型。 |
| 英文摘要: |
| Objective To establish a transgenic humanized ACE2 nude mouse model against a BALB/ c-nude mouse background. Methods Female human ACE2 (hACE2) transgenic mice were hybridized with male BALB/ c-nude mice to produce an F1 generation. An F2 generation was then obtained by backcrossing the F1 generation hACE2 mice with BALB/ c-nude mice, and F3 generation hACE2 transgenic nude mice were obtained by intercrossing the F2 generation hACE2 mice. Finally, F3 hACE2 transgenic nude mice were compared with C57BL/ 6J wild-type mice, hACE2 mice, and BALB/ c-nude mice in terms of their phenotypes, and physiological and immunological characteristics. Results (1)There were no significant differences between hACE2 transgenic nude mice and C57BL/ 6J wild-type mice, hACE2 mice, or BALB/ c-nude mice in terms of their phenotypes. (2) hACE2 transgenic nude mice had similar physiological indexes to BALB/ c-nude mice and lacked thymuses. However, the spleen and liver indexes differed significantly between hACE2 transgenic nude mice and BALB/ c-nude mice ( P< 0.05). Routine blood indexes also showed that the neutrophil, lymphocyte, and monocyte percentages differed significantly between hACE2 transgenic nude mice and C57BL/ 6J wild-type mice and hACE2 mice (P < 0. 01). (3)There were also significant differences in immunological indexes between hACE2 transgenic nude mice and C57BL/ 6J wild-type mice and hACE2 mice in terms of the percentages of CD3, CD4, CD11B, and natural killer cells (P< 0.01), but no significant differences between hACE2 transgenic nude mice and BALB/ c-nude mice. Conclusions We successfully established a line of hACE2 transgenic nude mice with a BALB/ c-nude mouse background. |
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