首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
沈利叶,潘永明,徐雁云,郁晨,黄俊杰,马全鑫,陈民利.高脂高糖饮食诱导五指山小型猪动脉粥样硬化模型肠道菌群的变化[J].中国实验动物学报,2022,30(3):299~308.
高脂高糖饮食诱导五指山小型猪动脉粥样硬化模型肠道菌群的变化
Changes in intestinal flora in a Wuzhishan minipig atherosclerosis model induced by high-fat and high-sugar diet
投稿时间:2021-09-25  
DOI:10. 3969 / j.issn.1005-4847. 2022. 03. 001
中文关键词:  肠道菌群  高脂高糖饮食  动脉粥样硬化模型  五指山小型猪
英文关键词:intestinal flora  high-fat and high-sugar diet  atherosclerosis model  Wuzhishan minipig
基金项目:
作者单位E-mail
沈利叶 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053 17805053349@ 163. com 
潘永明 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
徐雁云 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
郁晨 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
黄俊杰 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
马全鑫 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
陈民利 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053 cmli991@ zcmu. edu. cn 
摘要点击次数: 611
全文下载次数: 203
中文摘要:
       目的 观察高脂高糖饮食致五指山小型猪动脉粥样硬化( atherosclerosis,AS)模型肠道菌群的变化。 方法 雄性五指山小型猪 12 只,按体重分为两组,即正常对照(NC)组和模型(AS)组,每组 6 只。 NC 组饲喂正常饲料,AS 组饲喂高脂高糖饲料,连续饮食 24 周后,前腔静脉采血测定糖脂代谢等生化指标,同时进行葡萄糖耐量试验。实验结束后行安死术,观察体内脂肪沉积,并取主动脉血管行苏丹 IV 染色和腹主动脉血管行 HE 染色分别观察脂质沉积和组织病理学变化。取盲肠内容物进行 16S rDNA 测序分析肠道菌群的变化。 结果 与 NC 组比较, AS 模型组血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血糖(GLU)、果糖胺 (FMN)菌明显升高(P< 0.05,P< 0.01),体重和体内脂肪沉积亦显著增加(P< 0.01),同时 AS 模型组主动脉血 管明显脂质沉积和 AS 病变。 肠道菌群测序分析显示,与 NC 组相比,AS 模型组肠道菌群 α-多样性显著降低(P< 0.05,P< 0.01)。 在门水平上,AS 模型组拟杆菌门和放线菌门明显下降(P< 0.05,P< 0.01),变形菌门和厚壁菌 门/ 拟杆菌门比值均显著升高( P< 0.05)。 在属水平上,AS 模型组黑斯伯利亚菌属( Hespellia)、瘤胃球菌属 (Ruminococcus)、毛螺菌属 ( Lachnospiracea _ incertae _ sedis)、 假丁酸弧菌属 ( Pseudobutyrivibrio)、 巴恩斯氏菌属 (Barnesiella)等 17 个菌属的相对丰度显著下降(P< 0.05,P< 0.01),沙壤土杆菌属(Ramlibacter)、鞘氨醇杆菌属 (Sphingorhabdus)、污水管杆菌属(Cloacibacillus)、草酸杆菌属(Oxalobacter)、鞘脂单胞菌属( Sphingomonas)、新鞘脂菌属(Novosphingobium)等 11 个菌属的相对丰度显著上升(P< 0.05)。 功能预测分析显示,脂多糖生物合成、脂多糖生物合成蛋白途径、初级胆汁酸的生物合成、花生四烯酸代谢等多条途径可能参与 AS 的形成。 相关分析显示, TC、内中膜厚度、脂质沉积、果糖胺与草酸杆菌属、鞘氨醇单胞菌属、污水管杆菌属、梭菌属、瘤胃球菌属等 14 个菌属存在关联。 结论 高脂高糖饮食诱导的五指山小型猪 AS 模型具有肥胖、糖脂代谢紊乱和明显的 AS 病变的特点,其发生可能与肠道菌群失调有关。
英文摘要:
       Objective To observe changes in the intestinal flora in a Wuzhishan minipig model of atherosclerosis (AS) induced by a high-fat / high-sugar diet. Methods Twelve male Wuzhishan minipigs were divided into normal control (NC) and AS model groups according to body weight (n= 6 per group). The NC group was fed a normal diet and the AS model group was fed a high-fat / high-sugar diet. After 24 weeks of continuous diet, blood samples were taken from the anterior vena cava to determine biochemical indexes, including glucolipid metabolism, and a glucose to tolerance test was also performed. At the end of the experiment, the minipigs were euthanized and body fat deposition was observed. The aortic vessels were stained with Sudan IV and the abdominal aortic vessels were stained with hematoxylin and eosin to observe lipid deposition and histopathological changes, respectively. The cecal contents were used for 16S rDNA sequencing to analyze changes in the intestinal flora. Results Serum total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, blood glucose, and fructosamine levels were all significantly higher in the AS model group compared with the NC group (P< 0.05, P< 0.01). Body weight and body fat deposition were also significantly higher in the AS model group ( P< 0.01). The aortic vessels showed increased lipid deposition and AS lesions. DNA sequencing analysis showed significantly lower intestinal flora α-diversity in the AS model group compared with the NC group ( P< 0.05, P< 0.01). At the phylum level, the phyla Bacteroidetes and Actinomycetes were significantly reduced in the AS model group (P< 0.05, P< 0.01), while the phylum Proteobacteria and the Firmicutes/ Bacteroidetes ratio were significantly increased (P< 0.05). At the genus level, the relative abundances of 17 genera, including Hespellia, Ruminococcus, Lachnospiracea_incertae_sedis, Pseudobutyrivibrio, and Barnesiella, were significantly decreased (P< 0.05, P< 0.01), and the relative abundances of 11 genera, including Ramlibacter, Sphingorhabdus, Cloacibacillus, Oxalobacter, Sphingomonas, and Novosphingobium, were significantly increased (P< 0.05). Functional prediction analysis showed that multiple pathways such as lipopolysaccharide biosynthesis, lipopolysaccharide biosynthesis proteins, primary bile acid biosynthesis, and arachidonic acid metabolism may participate in the formation of AS. Correlation analysis showed that total cholesterol, intima media thickness, lipid deposition, and fructosamine were associated with 14 genera, including Oxalobacter, Sphingomonas, Cloacibacillus, Clostridialse _ unclassified, and Ruminococcus. Conclusions High-fat / high sugar diet-induced AS in Wuzhishan minipigs is characterized by obesity, disturbed glucolipid metabolism, and obvious AS lesions, possibly related to dysbiosis of the intestinal flora.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 3023965 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码