首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
张茹鑫,李承罡,杜若琛,原一桐,李宵,赵碧春,张玉娟,王春芳.人脐带间充质干细胞对自然衰老大鼠海马自噬水平的影响[J].中国实验动物学报,2020,28(6):796~804.
人脐带间充质干细胞对自然衰老大鼠海马自噬水平的影响
Effects of human umbilical cord mesenchymal stem cells on autophagy in the naturally aging rat hippocampus
投稿时间:2020-08-28  
DOI:10. 3969 / j.issn.1005-4847. 2020. 06. 009
中文关键词:  衰老  自噬  人脐带间充质干细胞  认知衰退  大鼠
英文关键词:aging  autophagy  human umbilical cord mesenchymal stem cells  cognitive decline  rats
基金项目:
作者单位E-mail
张茹鑫 山西医科大学实验动物中心,太原 030001 zhangruxinin@ 163.com 
李承罡 山西医科大学第二医院,太原 030001 lcgsydeyjzk@ 163.com 
杜若琛 山西医科大学实验动物中心,太原 030001  
原一桐 山西医科大学实验动物中心,太原 030001  
李宵 山西医科大学实验动物中心,太原 030001  
赵碧春 山西医科大学实验动物中心,太原 030001  
张玉娟 山西医科大学实验动物中心,太原 030001  
王春芳 山西医科大学实验动物中心,太原 030001 wangchunfang@ sxmu.edu.cn 
摘要点击次数: 188
全文下载次数: 219
中文摘要:
       目的 本研究探究移植人脐带间充质干细胞( human umbilical cord mesenchymal stem cells, hUCMSCs)是否能通过调节自然衰老大鼠自噬水平来改善学习记忆及认知衰退。 方法 SD 大鼠屏障环境饲养至衰老年龄(24 月龄),行为学挑选出认知衰退明显的老年鼠,分为细胞移植组(H 组)和生理盐水组(C 组),3 月龄 SD 大鼠为正常组(N 组)。 H 组:尾静脉注射 hUCMSCs 细胞混悬液 500 μL,剂量为每只 2 × 10P>6P>> 个,C 组和 N 组分别尾静脉注射无菌生理盐水。时间均为每周 1 次,连续 4 周。通过 Morris 水迷宫,Y 迷宫及新物体识别实验观察其学习记忆能力变化;通过 HE 染色观察大鼠海马神经元形态改变;通过 Western Blot 检测海马 LC3II/ I、Beclin1、P62 的相对表达量。 结果 (1)行为学结果显示 H 组和 N 组学习记忆能力显著高于 C 组。 (2)HE 染色中 H 组和 N 组海马 CA1 区和 DG 区神经细胞排列较规整,细胞结构完整,细胞核饱满,C 组 CA1 区神经元数量减少,细胞排列疏松,细胞核固缩,呈退行性病变。 (3)H 组和 N 组中 P62 蛋白含量显著低于 C 组,Beclin1 和 LC3Ⅱ/ Ⅰ 显著高于 C 组。 结论 静脉注射人脐带间充质干细胞改善自然衰老大鼠的学习记忆及认知衰退,可能与干预后自噬水平的恢复有关。
英文摘要:
       Objective To explore whether transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) can improve learning, memory and cognitive decline by regulating autophagy levels in naturally aging rats. Methods Sprague-Dawley rats were reared in a barrier environment to the age of 24 months. Behavioral studies select aging rats with obvious cognitive decline; the rats were divided into a cell transplantation group ( group H) and a normal saline group (group C). For comparison, a normal group ( group N) of 3-month-old rats was included. Rats in group H were injected with 2×10P>6P>> hUCMSCs in a volume of 500 μL through the tail vein. Rats in groups C and N were injected with an equal volume of sterile saline through the tail vein. Injections were performed once per week for 4 weeks. Changes in learning and memory ability were assessed using the Morris water maze, Y maze, and novel object recognition test. Changes in rat hippocampal neuron morphology were assessed using hematoxylin-eosin staining. Relative expression levels of hippocampal LC3II/ I, Beclin1, and p62 were assessed using Western Blot. Results Behavioral analyses showed that the learning and memory abilities were significantly better in groups H and N than in group C. HE staining of nerve cells in the CA1 and DG areas of the hippocampus revealed more regular arrangements, more complete cell structure, and more intact nuclei in groups H and N. Conversely, the number of neurons in the CA1 area was reduced, cell arrangement was loose, nuclei were shrunken, and degenerative disease was present in group C. p62 protein content was significantly lower in groups H and N than in group C, while Beclin1 and LC3II/ I protein levels were significantly higher in groups H and N than in group C. Conclusions Intravenous injection of hUCMSCs improved learning, memory, and cognitive decline in naturally aging rats, presumably by restoration of autophagy.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1937522 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码