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周康,叶妙勇,赵凡,马轲,吕伯东,许增宝.两种黄芪剂量补阳还五汤对海绵体神经损伤性大鼠勃起功能障碍的治疗效果[J].中国实验动物学报,2020,28(6):788~795.
两种黄芪剂量补阳还五汤对海绵体神经损伤性大鼠勃起功能障碍的治疗效果
Effect of the Astragalus dose in Buyang Huanwu decoction on erectile dysfunction in rats with cavernous nerve injury
投稿时间:2020-07-14  
DOI:10. 3969 / j.issn.1005-4847. 2020. 06. 008
中文关键词:  补阳还五汤  海绵体神经损伤  勃起功能障碍  黄芪  海绵体内压
英文关键词:Buyang Huanwu decoction  cavernous nerve injury  erectile dysfunction  Astragalus  intracavernous pressure
基金项目:
作者单位E-mail
周康 浙江中医药大学第二临床医学院,杭州 310053 844310446@ qq.com 
叶妙勇 温岭市第一人民医院泌尿外科,浙江 温岭 317500 yemiaoyong1993@ 163.com 
赵凡 南通大学附属医院泌尿外科/ 男科,江苏 南通 226001  
马轲 浙江中医药大学第二临床医学院,杭州 310053  
吕伯东 4. 浙江中医药大学附属第二医院泌尿男科,杭州 310005
5. 浙江省中医药重点实验室/ 男科实验室,杭州 310053
6. 浙江中医药大学泌尿男科研究所,杭州 310005 
lbd168@ 126.com 
许增宝 湖州市中医院,浙江 湖州 313000 1643394189@ qq.com 
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中文摘要:
       目的 探究两种黄芪剂量补阳还五汤对海绵体神经损伤性大鼠勃起功能障碍的治疗效果。 方法 将 32 只 SD 大鼠随机平均分成假手术组、模型组、30 g 组(模型 + 含黄芪 30 g 补阳还五汤)、120 g 组(模型 + 黄芪 120 g 补阳还五汤);通过手术对 SD 大鼠海绵体神经进行钳夹(但不剪断)以造成海绵体神经损伤所致勃起功能障碍(erectile dysfunction,ED)大鼠模型;术后 5 d 采用不同剂量黄芪(分别设置黄芪 30、120 g)的补阳还五汤灌胃干预,假手术组与模型组均予以等量生理盐水灌胃,每日 1 次,连续 30 d。 30 d 后测定大鼠阴茎海绵体内压 (intracavernous pressure,ICP),Masson 染色技术观察海绵体组织平滑肌与胶原沉积含量,免疫应荧光技术和 Western Blot 检 测 各 组 神 经 型 一 氧 化 氮 合 酶 ( neuronal nitric oxide synthase, nNOS), 神 经 丝 蛋 白 轻 链 多 肽 (neurofilament light polypeptide,NF-L),生长相关蛋白 43(growth associated protein 43,GAP-43)变化。 结果 与假手术组相比,模型组大鼠 ICP 明显降低(P< 0. 01),阴茎海绵体平滑肌面积/ 胶原面积减少(P< 0. 01),NF-L、nNOS、 GAP43 水平明显下降(P< 0. 01);与模型组相比,30 g 组与 120 g 组大鼠 ICP 明显增高(P< 0. 01),阴茎海绵体平滑 肌面积/ 胶原面积增高(P< 0. 01),nNOS、NF-L、GAP43 水平明显增高(P< 0. 01),其中与 30 g 组相比,120 g 组大鼠 ICP 明显增高(P< 0. 05),阴茎海绵体平滑肌面积/ 胶原面积增高(P< 0. 05),nNOS、NF-L、GAP43 水平明显增高 (P< 0. 01)。 结论 补阳还五汤可以明显改善大鼠勃起功能障碍,且《医林改错》原方所载的补阳还五汤治疗效果更优。
英文摘要:
       Objective To explore the therapeutic effects of two different doses of Buyang Huanwu decoction containing Astragalus on erectile dysfunction (ED) in rats with cavernous nerve injury. Methods Thirty-two Sprague- Dawley ( SD) rats were randomly divided into four groups: sham operation model, 30 g ( model + Buyang Huanwu decoction containing 30 g Astragalus) and 120 g (model + Buyang Huanwu decoction containing 120 g Astragalus). The cavernous nerve of the rats was surgically clamped (but not cut) to establish the rat model of ED caused by cavernous nerve injury. Five days after the operation, a 30 g or 120 g dose of Astragalus was used for the gavage intervention of Buyang Huanwu decoction, or the same volume of normal saline ( sham and model groups) was given, once a day for 30 consecutive days. After 30 days, the intracavernous pressure ( ICP) of the rat corpus cavernosum was measured. Masson staining was used to observe the content of smooth muscle and collagen deposition in the spongy tissue. Fluorescence and western blotting were used to detect changes in neuronal nitric oxide synthase ( nNOS), neurofilament light polypeptide (NF-L), growth associated protein 43 (GAP-43) expression in each group. Results Compared with the sham group, the model group showed significantly decreased ICP ( P < 0. 01), decreased area of penile smooth muscle / collagen ( P < 0. 01), and significantly decreased levels of NF-L, nNOS and GAP43 (P< 0. 01). Compared with the model group, the rats in the 30 g and 120 g Astragalus groups showed significantly increased ICP (P < 0. 01), increased area of penile smooth muscle / collagen (P< 0. 01), and significantly increased levels of nNOS, NF-L and GAP43 (P< 0. 01). Compared with the 30 g group, the rats in the 120 g Astragalus group showed significantly increased ICP (P< 0. 05), increased area of penile smooth muscle / collagen (P< 0. 05), and significantly increased levels of nNOS, NF-L and GAP43 (P< 0. 01). Conclusions Buyang Huanwu decoction containing Astragalus obviously improved ED in cavernous nerve injury model rats. The higher dose of Astragalus (120 g), which is used in the original prescription of “Medical Forest Correction”, was more efficacious.
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