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王子,王雪峰,吴振起.清肺透邪汤介导 NLRP3 炎性小体抑制 NF-κB 信号通路改善肺炎支原体小鼠炎性反应[J].中国实验动物学报,2020,28(4):470~477.
清肺透邪汤介导 NLRP3 炎性小体抑制 NF-κB 信号通路改善肺炎支原体小鼠炎性反应
Qingfei Touxie decoction mediates NLRP3 inflammasome inhibition of the NF-κB signaling pathway and improves inflammatory responses in mice with Mycoplasma pneumoniae
投稿时间:2020-03-23  
DOI:10. 3969 / j.issn.1005-4847. 2020. 04. 006
中文关键词:  清肺透邪汤  肺炎支原体肺炎  炎性小体  NLRP3
英文关键词:Qingfei Touxie decoction  mycoplasma pneumoniae pneumonia  inflammasome  NLRP3
基金项目:
作者单位E-mail
王子 辽宁中医药大学研究生学院,沈阳 110847 wangzi52617@ 163.com 
王雪峰 辽宁中医药大学附属医院,沈阳 110032  
吴振起 辽宁中医药大学附属医院,沈阳 110032 lnzywxf@ 163.com 
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中文摘要:
      目的 探讨清肺透邪汤对肺炎支原体肺炎(MPP)小鼠的治疗作用及其作用机制。 方法 40 只雄 性 SPF 级 BALB/ c 小鼠随机分为对照组、模型组、清肺透邪汤低、中及高剂量组,每组 8 只。 利用鼻腔滴注肺炎支原 体菌液(MP)的方法建立肺炎支原体肺炎小鼠模型,模型组小鼠灌服生理盐水,清肺透邪汤低、中、高剂量组小鼠分 别按 200、400、800 mg / kg 体重每天剂量灌服清肺透邪汤混悬液。 治疗两周后,计算小鼠肺组织指数;HE 染色检测肺组织病理改变;TUNEL 检测小鼠肺内细胞凋亡情况;ELISA 检测各小鼠肺泡灌洗液中 IL-1β、TNF-α、IL-6、IL-18 含量;qPCR 检测肺组织中 NLRP3、MyD88 及 NF-κB mRNA 的表达;Western Blot 检测肺组织中 NLRP3、MyD88 及 p-NF-κB蛋白的表达。 结果 清肺透邪汤显著降低 MPP 小鼠肺指数,减轻肺病理损伤和细胞凋亡率(P< 0. 05);清 肺透邪汤还可显著降低肺泡灌洗液中 IL-1β、TNF-α、IL-6、IL-18 水平(P< 0. 05);并降低 NLRP3、MyD88 和 NF-κB mRNA 和蛋白表达(P< 0. 05)。 结论 清肺透邪汤改善肺炎支原体肺炎小鼠肺损伤,其作用机制可能与抑制 NLRP3 炎性小体及 NF-κB 信号通路有关。
英文摘要:
      Objective To explore the therapeutic effect and mechanism of Qingfei Touxie decoction on mice with Mycoplasma pneumoniae (MP). Methods Forty male specific-pathogen-free BALB/ c mice were randomly divided into the control, model, low-dose, medium-dose, and high-dose Qingfei Touxie decoction groups, with n = 8 mice per group. A mouse model of MPP was established via nasal instillation of solution containing MP. Mice in the model and control groups received 0. 9% saline solution by oral gavage, and mice in the Qingfei Touxie decoction (QFTXT)-treated groups received QFTXT at low (200 mg / kg body weight), medium (400 mg / kg body weight) and high (800 mg / kg body weight) doses daily. After 2 weeks of treatment, the lung tissue indices of the mice were calculated, and the pathological changes in the lung tissue were observed via hematoxylin and eosin staining. Apoptosis in the mouse lung cells was detected via TUNEL staining, and the interleukin (IL)-1β, TNF-α, IL-6, and IL-18 levels were detected using enzyme-linked immunosorbent assays. NLRP3, MyD88 and NF-κB mRNA expressions in the lung tissue were detected using qPCR, and the NLRP3, MyD88 and p-NF-κB protein expressions in the lung tissue were detected via western blot. Results Qingfei Touxie decoction significantly reduced the lung indices of the MPP mice and reduced the lung pathological damage and apoptotic rates (P< 0. 05). Qingfei Touxie decoction also significantly reduced the IL-1β, TNF-α, IL-6 and IL-18 levels ( P< 0. 05) and NLRP3, MyD88 and NF-κB mRNA and protein expressions ( P< 0. 05). Conclusions Qingfei Touxie decoction attenuated lung injuries in mice with MP pneumonia, possibly by inhibiting NLRP3 inflammatory bodies and the NF-κB signaling pathway.
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