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姚一博.盐酸洛哌丁胺诱导的小鼠慢传输型便秘模型的实验研究[J].中国实验动物学报,2020,28(3):370~375.
盐酸洛哌丁胺诱导的小鼠慢传输型便秘模型的实验研究
Experimental study of slow transit constipation induced by loperamide hydrochloride in mice
投稿时间:2019-12-26  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 012
中文关键词:  盐酸洛哌丁胺  慢传输型便秘  小鼠模型
英文关键词:loperamide hydrochloride  slow transit constipation  mouse model
基金项目:
作者单位E-mail
姚一博 上海中医药大学附属龙华医院肛肠科,上海 200032 elevenzoe@ 163.com 
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中文摘要:
      目的 通过盐酸洛哌丁胺诱导慢传输型便秘小鼠模型,比较不同剂量、不同给药时间所诱导模型之间的差异,探索建立稳定的盐酸洛哌丁胺诱导的慢传输便秘小鼠模型各项实验参数。 方法 采用健康雄性 C57BL/ 6 小鼠,配制 0. 025、0. 25 和 2. 5 mg / mL 的盐酸洛哌丁胺混悬液灌胃,给药时间为每日上午 9 时和下午 3 时, 分两次给药。从肠道推进率检测、排便量检测等指标进行造模效果评价,观察不同剂量、不同给药时间对于慢传输型便秘小鼠模型的影响。 结果 1)对比不同剂量的盐酸洛哌丁胺造模的效果,在 0. 5 mg / kg ~ 50 mg / kg 的给药浓 度范围内,通过比较组间肠道推进率,结果表明 50 mg / kg 组与空白组相比,小鼠肠道推进率显著降低、排便量明显 减少,差异具有统计学意义(P < 0. 05);(2)对比 3、7、10 和 14 d 四个不同造模天数,造模第 7 天时,小鼠的肠道推 进率与空白组相比明显降低(P < 0. 05);而 3、10、14 d 肠道推进率相比无明显变化(P > 0. 05)。 结论 本次研究发 现采用 50 mg / kg 的剂量连续灌胃 7 d,可使小鼠的肠道推进率显著降低,排便量明显减少,能够制造相对稳定的结肠传输障碍性便秘小鼠模型,为盐酸洛哌丁胺诱导慢传输型便秘模型的探索提供了有价值的数据资料。
英文摘要:
      Objective To find a stable method for establishing a loperamide hydrochloride-induced slow transit constipation (STC) model by comparing the differences among STC mouse models established by different loperamide hydrochloride concentrations and administration times. Methods Healthy male C57BL/ 6 mice were given 0. 025, 0. 25 or 2. 5 mg / mL loperamide hydrochloride by intragastric administration twice a day at 9:00 and 15:00. The small intestinal propulsion rate, defecation quantity and other indicators were used to evaluate the effects of different doses and administration times on the STC mouse model. Results ( 1) Comparing the effects of different dosages of loperamide hydrochloride in the range of 0. 5 mg / kg to 50 mg / kg revealed that the small intestinal propulsion rate and defecation quantity significantly decreased in the 50 mg / kg dosage group compared with those in the control group (P < 0. 05). (2) At day 7 after oral administration of loperamide hydrochloride, the small intestinal propulsion rate significantly decreased compared with that in control group (P < 0. 05), whereas this rate did not significantly change at days 3, 10 and 14 (P> 0. 05). Conclusions The small intestinal propulsion rate and defecation quantity significantly decreased after continuous administration of 50 mg / kg loperamide hydrochloride for 7 days. Hence, this is a relatively stable method for establishing an STC mouse model. This study provides valuable data for the exploration of the loperamide hydrochloride-induced STC model.
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