首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
马全鑫.新型高脂血症易感(WSHc)大鼠非酒精性脂肪肝病模型的建立与病理特点研究[J].中国实验动物学报,2020,28(3):314~322.
新型高脂血症易感(WSHc)大鼠非酒精性脂肪肝病模型的建立与病理特点研究
Establishment and pathological characteristics of a non-alcoholic fatty liver disease model in a new type of hyperlipidemia-susceptible (WSHc) rats
投稿时间:2019-11-21  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 004
中文关键词:  高脂血症易感大鼠,WSHc 大鼠,非酒精性脂肪肝病,动物模型
英文关键词:hyperlipidemia susceptible rats, wshc rats, non-alcoholic fatty liver disease, animal model
基金项目:
作者单位E-mail
马全鑫 浙江中医药大学,动物实验研究中心/ 比较医学研究所,杭州 310053 mqx1025@ hotmail.com 
摘要点击次数: 158
全文下载次数: 630
中文摘要:
      目的 利用一种新型的高脂血症易感(WSHc)大鼠建立非酒精性脂肪肝病(NAFLD)模型,观察 其血液生化指标变化及病理特点,探讨其应用与科研价值,为 NAFLD 疾病动物模型提供新的选择。 方法 20 只 7 ~ 8周龄的 WSHc 大鼠,随机平均分为 2 组,分别饲喂普通饲料和高脂饲料;另取 20 只同周龄的 Wistar 大鼠,采 取相同处理方式分组作为品系对照。 持续饲喂 12 周后,检测血清 TC、TG、ALT 和 AST 水平,并进行弹性超声成 像。 处死动物后取肝进行 HE、油红 O、Masson 和免疫荧光染色,观察各组大鼠肝大体细胞形态、脂质沉积、炎症 反应和纤维化情况,分析高脂饲喂后 WSHc 大鼠肝的病变特点。 结果 与饲喂高脂饲料的 Wistar 大鼠比较, WSHc 大鼠经高脂诱导后,血清总胆固醇显著升高并与临床高脂血症患者接近;肝脂质异常沉积并形成大泡性 脂滴;脂滴周围有炎症细胞浸润形成炎症灶,巨噬细胞增多;可见散在的纤维化病变。 然而,这些病理变化在高 脂诱导的 Wistar 大鼠肝组织中并未发现。 结论 相较于 Wistar 大鼠, WSHc 大鼠高脂诱导后血脂与肝酶接近临 床水平,肝组织中有更严重的脂质沉积和炎症反应,且伴有散在的肝纤维化,因此可作为一种新的、理想的 NAFLD 动物模型。
英文摘要:
      Objective To establish a non-alcoholic fatty liver disease (NAFLD) rat model in a new type of hyperlipidemia (WSHc) rat. We observed the pathological characteristics of the NAFLD model and explored possible research applications, as well as new options for animal models of NAFLD. Methods 20 WSHc rats aged 7 – 8 weeks were randomly divided into 2 groups according to whether they would receive a common diet or high-fat diet. Another 20 Wistar rats of the same age were then grouped as a strain control, and received the same treatment. After 12 weeks of continuous feeding, blood samples were taken to detect blood biochemical indicators and elastic ultrasound imaging was performed. After execution of the animals, the liver tissue was subjected to HE, oil red “ O ”, Masson, and immunofluorescent staining, and the general cell morphology, lipid performance, inflammatory response, and liver fibrosis stage in each group were observed. The lesion characteristics of the liver of WSHc rats after a high-fat diet were analyzed. Results Compared with Wistar rats on a high-fat diet, WSHc rats showed a significant increase in serum total cholesterol, similar to that seen in patients with clinical hyperlipidemia. We also found that liver lipids were abnormally deposited to form lipid droplets, and that inflammatory cells positioned around the lipid droplets formed an inflammatory lesion. Further, we observed an increase in macrophages and instances of scattered fibrosis. These pathological changes were not found in the liver tissue of high-fat-exposed Wistar rats. Conclusions Compared with conventional rats, WSHc rats on a high-fat diet represent a more clinically similar NAFLD animal model, which is characterized by blood biochemical indicators, a severe inflammatory response, and sporadic liver fibrosis approaching clinical levels.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1669124 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码