Observation of evaluation points for blood glucose intervention in a mouse model of type 2 diabetes mellitus
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Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Basic Medical College, Lanzhou University, Lanzhou 730000, China
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摘要:
目的 观察 2 型糖尿病小鼠模型血糖变化规律,探讨餐后 2 h 血糖作为血糖干预评价点的可行性。 方法 选取健康 C57BL/ 6 小鼠, 用高脂饮食加链脲佐菌素( STZ)诱导 2 型糖尿病(T2DM)实验组,并设立正常对照。分别测定两组连续空腹血糖及连续随机血糖,分析小鼠血糖规律,评估餐后 2 h 血糖作为 T2DM 小鼠血糖干预评价点的科学性;通过长期观察糖尿病小鼠餐后 2 h 血糖波动,验证餐后 2 h 血糖干预评价点的稳定性。 结果 T2DM 小鼠连续空腹血糖显示,餐后空腹 2 ~ 3 h 期间血糖变化幅度相对稳定,禁食超过 4 h 后,T2DM 组小鼠血糖 继续大幅度降低,而正常组却能缓慢上升,并保持血糖相对稳定;7 周内 T2DM 小鼠餐后2 h血糖波动显示,餐后 2 h 血糖稳定。 结论 餐后 2 h 血糖能稳定地反映 T2DM 小鼠模型的血糖变化规律,具有较好的血糖干预实验评价应 用价值。
Abstract:
Objective To observe changes of blood glucose in mice with type 2 diabetes mellitus (T2DM), and to explore the feasibility of using 2-hour postprandial blood glucose as an evaluation point of blood glucose intervention. Methods T2DM mice induced by a high-fat diet and streptozotocin ( STZ) were the experimental group and normal C57BL/ 6 mice were used as the control group. Continuous fasting blood glucose and continuous random blood glucose were measured in the two groups, and the blood glucose levels in the mice were analyzed to evaluate the use of 2-hour postprandial blood glucose as an evaluation point of blood glucose intervention in T2DM mice. The stability of the 2-hour postprandial blood glucose evaluation point was verified by the long-term observation of 2-hour postprandial blood glucose fluctuation in diabetic mice. Results The continuous fasting blood glucose of T2DM mice was significantly reduced after fasting for more than 4 hours. In contrast, blood glucose changes in T2DM mice at 2- 3 hours after eating a meal were relatively stable, which might reflect the stability of the model over a long period. For 7 weeks, the 2-hour postprandial blood glucose level of T2DM mice showed it was stable at 2 hours after meals. Conclusions The 2-hour postprandial blood glucose level reflects the stable blood glucose change in T2DM mice, which might have value for the evaluation of blood glucose intervention experiments.