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黄文亭,曾子纯,王冬菊,肖小敏.改良减少子宫血流灌注法建立子痫前期大鼠模型[J].中国实验动物学报,2019,27(6):776~780.
改良减少子宫血流灌注法建立子痫前期大鼠模型
Establishment of a rat model of pre-eclampsia via the improved method of reduced-uterine perfusion pressure
投稿时间:2019-06-03  
DOI:10. 3969 / j.issn.1005-4847. 2019. 06. 014
中文关键词:  先兆子痫  建模  动物模型  大鼠
英文关键词:pre-eclampsia  modeling  animal model  rat
基金项目:
作者单位E-mail
黄文亭 暨南大学附属第一医院, 广州 510632 1415430421@ qq.com 
曾子纯 暨南大学附属第一医院, 广州 510632 857392133@ qq.com 
王冬菊 暨南大学附属第一医院, 广州 510632  
肖小敏 暨南大学附属第一医院, 广州 510632 xiaoxiaomin55@ hotmail.com 
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中文摘要:
      目的 探讨子痫前期(PE)大鼠模型的建立方法?方法 SPF 级妊娠Wistar 大鼠39 只,其中减少子宫血流灌注(RUPP)组16 例?RUPP+灌水组10 例?假手术组13 例?分别孕14 d 实施RUPP 手术或假手术,孕18 d监测血压?尿蛋白及仔鼠状态?结果 (1)RUPP 手术对孕鼠的影响:与假手术组比较,RUPP 组及RUPP+灌水组孕鼠收缩压?舒张压及平均动脉压均有不同程度上升,但单纯RUPP 组血压上升不稳定,舒张压与假手术组差异无统计学意义;RUPP+灌水组血压稳定上升,且显著高于单纯RUPP 组;RUPP 组较假手术组孕18 d,24 h 尿蛋白定量显着增高?(2)RUPP 手术对仔鼠的影响:RUPP 手术使孕20 d 活产率及成仔率显著降低;RUPP 手术使胎仔体重及胎盘湿重显着低于假手术组,其中RUPP + 灌水组最明显?结论 RUPP + 灌水方法可诱发大鼠产生稳定的PE临床表现,推荐成为改良的RUPP 造模方法?
英文摘要:
      Objective To establish a rat model of pre-eclampsia. Methods Thirty-nine specific pathogen-free pregnant Wistar rats were divided into 3 groups, with 16 in the reduced-uterine perfusion pressure (RUPP) group, 10 in the RUPP+H2O group and 13 in the sham-operation group. RUPP or sham surgery were performed on the 14th gestational day (G14). Blood pressure, urine protein and fetal status were measured on G18. Results (1)Compared with the shamoperation group, the systolic blood pressure, diastolic blood pressure and mean arterial pressure in the RUPP and RUPP+ H2O groups were increased to different degrees in the pregnant rats, but the blood pressure in the RUPP group was unstable. The diastolic blood pressure did not significantly differ between the RUPP and sham-operation groups. The blood pressure of the RUPP+H2O group increased steadily and was significantly higher than that of the RUPP group. The 24-hour urine protein levels were significantly higher in the RUPP group than in the sham-operation group on G18.(2) RUPP surgery significantly reduced the live birth and formation rates on G20. The RUPP operation significantly reduced the fetal weight and placental wet weight compared with those of the sham-operation group, and these effects were the strongest in the RUPP+H2O group. Conclusions The RUPP+H2O method induces a stable clinical manifestation of pre-eclampsia in rats and is recommended as a modified method for modeling RUPP.
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