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李媛媛,周海燕,吴绿英,莫贤炜,李晶.大鼠高尿酸血症模型的建立与研究[J].中国实验动物学报,2019,27(6):747~752.
大鼠高尿酸血症模型的建立与研究
Establishment and study of a hyperuricemia rat model
投稿时间:2019-05-13  
DOI:10. 3969 / j.issn.1005-4847. 2019. 06. 009
中文关键词:  氧嗪酸钾  高尿酸血症  动物模型
英文关键词:potassium oxonate  hyperuricemia  animal model  rat
基金项目:
作者单位E-mail
李媛媛 华南理工大学生物科学与工程学院,广州 510006 670267110@ qq.com 
周海燕 华南理工大学生物科学与工程学院,广州 510006  
吴绿英 华南理工大学生物科学与工程学院,广州 510006  
莫贤炜 华南理工大学生物科学与工程学院,广州 510006  
李晶 华南理工大学生物科学与工程学院,广州 510006 lij@ scut.edu.cn 
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中文摘要:
      目的 建立一种大鼠高尿酸血症模型,并探讨高尿酸血症是否会产生继发性心血管疾病?方法将SPF 级雄性SD 大鼠32 只随机分成正常对照组(Group C),氧嗪酸钾模型组(Group M1),氧嗪酸钾联合高糖高脂饲料模型组(Group M2),氧嗪酸钾联合酵母膏饲料模型组(Group M3),每组8 只,连续造模3 周?通过比较大鼠的血清尿酸(uric acid,UA),尿素氮(blood urea nitrogen,BUN),肌酐酸(creatinine,Cr),胰岛素(insulin,INS),血糖(blood glucose,GLU),甘油三酯(triglyceride,TG)等指标,并辅以病理学检查结果来对大鼠高尿酸血症模型进行研究?结果 给予750 mg/ kg 体重氧嗪酸钾灌胃联合酵母膏饲料复制出的高尿酸血症模型大鼠,具有UA 水平显著升高( P <0. 01)?维持时间长?3/8 大鼠的肾发生病变的特点,在产生高尿酸血症的同时还伴有GLU?INS 和TG 水平改变及3/8 大鼠心脏的病变,提示继发性心血管病变的发生?结论 氧嗪酸钾灌胃联合酵母膏饲料造模方法较单纯氧嗪酸钾造模更适用于大鼠长期高尿酸血症模型的建立,同时还伴随着继发性血糖紊乱的发生,因此该造模方案可用于高尿酸血症与心血管疾病的相互干预机制研究的动物模型,并进一步应用于高尿酸血症的治疗药物的临床前药效学综合评价?
英文摘要:
      Objective To establish a rat model of hyperuricemia, and explore the possible secondary cardiovascular disease that could be induced by hyperuricemia in the model rats. Methods 32 male SD rats were randomly divided into control group (Group C), potassium oxonate model group (Group M1), potassium oxonate combined with high-sugar model group (Group M2), potassium oxonate combined with yeast extract feed model group (Group M3), 8 rats were treated in each group for 3 weeks. At the end of the experiment, the serum levels of uric acid (UA), blood urea nitrogen, creatinine, insulin (INS), blood glucose (GLU) and triglyceride (TG) were measured, and histopathological examination of liver, renal, and heart tissue was performed. Results Compared with the control group, a significant increase in serum levels of UA ( P <0. 01) and the number of rats showing renal lesions was found in the experimental group (3/8, 37. 5%) after oral administration of potassium oxonate at a dose of 750 mg/ kg, combined with yeast extract feed (Group M3). Also, the changes in GLU, INS, TG and the histopathology of heart tissue (3/8, 37. 5%) suggesting that rats in this group had secondary cardiovascular alterations. Conclusions Compared with the basic potassium oxonate model, the model of potassium oxonate combined with yeast extract feed is more suitable for the study of rat chronic hyperuricemia, accompanied by glucose metabolic impairment. It can also be used in rat models to establish mutual intervention mechanisms between hyperuricemia and cardiovascular disorders, and be applied in comprehensive preclinical pharmacodynamic evaluation of therapeutic drugs for hyperuricemia.
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