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苏先芝,刘一斐,孔文娟,闫菲,王晓梅,韩福国,刘清飞,史云.三种早发性卵巢功能不全小鼠造模方法的比较研究[J].中国实验动物学报,2019,27(6):725~732.
三种早发性卵巢功能不全小鼠造模方法的比较研究
Comparison of three methods for the establishment of mouse models of premature ovarian insufficiency
投稿时间:2019-04-25  
DOI:10. 3969 / j.issn.1005-4847. 2019. 06. 006
中文关键词:  早发性卵巢功能不全  二氧化乙烯基环己烯  造模方法  雷公藤  半乳糖  小鼠
英文关键词:premature ovarian insufficiency(POI)  vinylcyclohexene dioxide(VCD)  mouse models  establishing method  tripterygium glycosides(TG)  D-(+)-galactose(D-gal)
基金项目:
作者单位E-mail
苏先芝 北京中医药大学第一临床医学院,北京 100029  
刘一斐 北京中医药大学第一临床医学院,北京 100029  
孔文娟 北京中医药大学第一临床医学院,北京 100029  
闫菲 北京中医药大学第一临床医学院,北京 100029  
王晓梅 北京中医药大学第一临床医学院,北京 100029  
韩福国 清华大学药学院,北京 100084  
刘清飞 清华大学药学院,北京 100084 liuqf@ tsinghua.edu.cn 
史云 北京中医药大学东直门医院,北京 100700 zysyun@ 163.com 
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中文摘要:
      目的 对比分析早发性卵巢功能不全(POI)小鼠模型三种常用造模方法的差异,探索符合POI 临 床特征的造模方法?方法 将96 只动情周期规律的ICR 小鼠随机分为4 组:A 对照组?B 雷公藤组?C 二氧化乙烯 基环己烯(VCD)组?D 半乳糖组?适应性喂养后,分别给予不同造模干预,分别于干预后第16?46 和76 天随机处死 各组8 只小鼠,检测小鼠血清性激素?观察卵巢和子宫病理改变等?结果 各模型组小鼠E2?卵泡数与对照组有显 著差异;各模型组均出现动情周期紊乱,对照组则阴性?结论 VCD 腹腔注射法可损伤小鼠卵巢功能,造成小鼠 POI 模型;此造模方法简便易行?成功率高,在三种造模方法中成模更为理想,可推广?
英文摘要:
      Objective To evaluate the differences between three commonly used methods for establishing mouse models of premature ovarian insufficiency (POI), and to determine the method that is most accordant with the clinical features of POI. Methods Ninety-six ICR mice with regular estrous cycles were randomly divided into 4 groups: the control group (A), the tripterygium glycosides (TG) group (B), the 4-vinylcyclohexene diepoxide (VCD) group (C), and the D-(+)-galactose (D-gal) group (D). After intervention with the different chemicals, eight mice in each group were sacrificed, the serum sex hormone levels were measured, and the pathological changes of uterus and ovaries were examined. Results The level of E2 and the number of follicles differed significantly between mice in the model groups and those in the control group ( P <0. 01). And the estrous cycles in mice of the model groups were significantly irregular whereas those of the control group were regular. Conclusions Mouse models of POI can be successfully established with VCD via intraperitoneal injection. This method has the advantages of being an easier operation and having a higher success rate, as compared with the other two models method. It is the ideal one of the three establishing method and is suitable for popular use.
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