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黄忠强,刘书华,关雅伦,李韵峰,李雪娇,黄韧,张钰,李舸.非人灵长类单侧脑损伤模型运动感知行为的评价研究[J].中国实验动物学报,2019,27(5):577~582.
非人灵长类单侧脑损伤模型运动感知行为的评价研究
Investigation of evaluation method for motion and perception behavior in a nonhuman primate unilateral brain injury model
投稿时间:2019-02-21  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 005
中文关键词:  非人灵长类  单侧脑损伤  行为评价
英文关键词:nonhuman primate  unilateral brain injury  behavioral evaluation
基金项目:
作者单位E-mail
黄忠强 广东省实验动物重点实验室广东省实验动物监测所,广州 510663 1125385103@ qq.com 
刘书华 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
关雅伦 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
李韵峰 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
李雪娇 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
黄韧 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
张钰 广东省实验动物重点实验室广东省实验动物监测所,广州 510663  
李舸 广东省实验动物重点实验室广东省实验动物监测所,广州 510663 gonanlee@ gdlami.com 
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中文摘要:
      目的 通过评价大脑感知和运动行为反应相关脑区功能改变,是脑科学研究中基于非人灵长类开展脑重大疾病临床转化研究的重要策略,对解析病生理情况下大脑功能环路调控机制具有重要的意义?方法 选取7 ~ 9 岁雄性食蟹猴,通过构建右侧颈内动脉注射MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)诱导帕金森模型和左侧大脑中动脉闭塞所致缺血性脑卒中模型,利用自制的山和谷阶梯任务装置,评价大脑单侧脑区损伤对对侧感知和运动功能的影响?结果 正常猴在连续10 d 的山阶梯任务和谷阶梯任务中,左右手均能够达到满分;通过右侧颈内动脉注射MPTP 构建猴帕金森模型后,PET-CT 影像分析表明,3 只实验猴均出现右侧大脑黑质区多巴胺能神经元受损,在山阶梯任务和谷阶梯任务中,2 只实验猴出现左手运动功能显著损伤,空间识别能力基本正常,而1 只实验猴不仅出现左手运动能力损伤,可能也存在空间识别能力异常或右手运动能力损伤;通过电凝法构建左侧大脑中动脉闭塞所致缺血性脑卒模型猴,MRI 影响分析表明,实验猴右侧大脑出现典型的缺血梗死灶,在山阶梯任务和谷阶梯任务中,实验猴出现右手运动功能损伤,但空间识别能力基本正常?结论 山和谷阶梯任务实验能有效地应用于实验猴单侧脑损伤动物模型的运动感知行为评价?
英文摘要:
      Objective Evaluating motion and perception behavior to investigate the functional changes of brainregions in non-human primates is an important strategy to develop clinical transformation study for major diseases in brainscience research. Methods In this study, 7- to 9-year-old male cynomolgus monkeys were used to establish a Parkinson’smodel induced by right internal carotid artery injection of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and anischemic stroke model induced by left middle cerebral artery occlusion. The testing apparatus for hill and valley staircasetasks were used to evaluate the effect of unilateral brain injury on contralateral perception and motion function. Results Ina 10-day evaluation for hill and valley staircase tasks, full scores were marked for the left and right hands of normalmonkeys. After injection of MPTP into the right internal carotid artery, three experimental monkeys showed damageddopaminergic neurons in the right substantia nigra by PET-CT image analysis. In hill and valley staircase tasks, the lefthand motor function of two experimental monkeys was significantly injured, and the spatial recognition ability was normal.One experimental monkey not only had left hand motor ability damage but also showed abnormal spatial recognition ability orright hand motor ability. After left middle cerebral artery occlusion by electrocoagulation, typical ischemic infarctionappeared in the right brain in the experimental monkey in MRI analysis. In hill and valley staircase tasks, the right handmotor function of the experimental monkey was impaired, but the spatial recognition ability was normal. Conclusions Thehill and valley staircase tasks can be effectively applied to evaluate motion and perception behavior in a unilateral brain injury non-human primate animal model.
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