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赵晨,赵娇,赵晓波,白燕,金润铭.复方太子参止咳益气散治疗大鼠哮喘的机制探讨[J].中国实验动物学报,2019,27(4):532~539.
复方太子参止咳益气散治疗大鼠哮喘的机制探讨
Exploration of the mechanism of Taizishen Zhike Yiqi Powder in the treatment of asthma in rats
投稿时间:2019-04-17  
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 017
中文关键词:  复方太子参止咳益气散  哮喘  Th 细胞  细胞因子  氧化应激  大鼠
英文关键词:Taizishen Zhike Yiqi powder  asthma  T helper cell  cytokine  oxidative stress  rats
基金项目:
作者单位E-mail
赵晨 华中科技大学同济医学院附属协和医院儿科,武汉 430022 zhaochen2015@ hust.edu.cn 
赵娇 华中科技大学同济医学院附属协和医院儿科,武汉 430022  
赵晓波 华中科技大学同济医学院附属协和医院儿科,武汉 430022  
白燕 华中科技大学同济医学院附属协和医院儿科,武汉 430022 yanbaixh@ hust.edu.cn 
金润铭 华中科技大学同济医学院附属协和医院儿科,武汉 430022  
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中文摘要:
      目的 探究复方太子参止咳益气散(TZY)改善支气管哮喘大鼠症状的作用及机制。方法 大鼠随机分为7 组,分别为对照组,哮喘模型(OVA 诱导)组,TZY 低、中、高剂量灌胃+哮喘模型组,普米克令舒雾化+哮喘模型组,普米克令舒雾化+TZY 灌胃+哮喘模型组。HE 染色检测各组大鼠支气管黏膜下炎性细胞浸润;采用流式细胞术检测各组Th1/ Th2 细胞比例;ELISA 检测各组肺泡盥洗液(BALF)中IL-4、IL-5、IgE、IFN-γ 水平;生化检测各组BALF 中SOD、MDA、GSH 和T-AOC 的水平。结果 与哮喘组比较,TZY 中高剂量组及联用普米克令舒组Th1/ Th2细胞比例显著上升,IL-4、IL-5、IgE 水平显著下降,同时IFN-γ 水平显著升高( P <0. 05),且呈剂量依赖性;与哮喘组相比,TZY 中高剂量组及联用组GSH、T-AOC、SOD 水平显著升高,MDA 水平显著降低( P <0. 05)。与单用普米克令舒雾化相比,TZY 及普米克令舒联用组各项检测指标有明显差异( P <0. 05)。结论 TZY 可缓解大鼠支气管黏膜炎症状态,其机制可能与上调Th1/ Th2 细胞比例,调控炎症因子及氧化应激有关。
英文摘要:
      Objective To explore the effect and mechanism of formula TaizishenZhikeYiqi Powder (TZY) onimproving the symptoms of bronchial asthma rats. Methods Rats were randomly divided into 7 groups: control group,asthma model (OVA inducted), low dose group of traditional Chinese medicine, medium dose group of traditional Chinesemedicine, high dose group of traditional Chinese medicine, Pulmicort respules group, group of Pulmicort respules andtraditional Chinese medicine. HE staining was used to detect the submucocal inflammatory cell infiltration in the bronchialmucosa. Flow cytometry was used to detect the ratio of Th1 to Th2 cells in each group. The levels of IL-4, IL-5, IgE andIFN-γ in bronchoalveolar lavage fluid (BALF) of each group were detected by ELISA. The levels of SOD, MDA, GSH andT-AOC in BALF of each group were detected by biochemistry. Results TZY reduced airway inflammation exhibiting atherapeutic effect on asthma in the rats. Compared with the asthma group, the ratio of Th1 to Th2 cells in the TZY groupand joint use group increased significantly, the levels of IL-4, IL-5 and IgE decreased significantly, but the level of IFN-γincreased significantly ( P <0. 05) in a dose-dependent manner. Compared with the asthma group, the levels of GSH, T中AOC and SOD in the TZY groups and joint drug use group were significantly increased, and the level of MDA wassignificantly decreased. The TZY and Pulmicort respules combination group exerted similar or better effects than thePulmicort respules atomization. Conclusions TZY can alleviate the inflammatory state of bronchial mucosa in rats. Themechanism may be related to up-regulating the ratio of Th1 to Th2 cells, regulating inflammatory factors and oxidativestress.
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