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张飞燕,金洁,刘超,王芸,谢丽分,张晓迪,邬继文,吕龙宝.基于高通量测序技术测定普通棉耳狨猴粪便微生物多样性[J].中国实验动物学报,2019,27(3):366~373.
基于高通量测序技术测定普通棉耳狨猴粪便微生物多样性
Analysis of microbial diversity in feces of the common cotton-eared marmosets by high-throughput sequencing
投稿时间:2018-12-07  
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 015
中文关键词:  普通棉耳狨猴  粪便  高通量测序  物种组成  优势菌群
英文关键词:common cotton-eared marmoset  feces  Illumina MiSeq  species composition  microbial diversity
基金项目:
作者单位E-mail
张飞燕 中国科学院昆明动物研究所,昆明 650000 1175905001@ qq.com 
金洁 中国科学院昆明动物研究所,昆明 650000  
刘超 中国科学院昆明动物研究所,昆明 650000  
王芸 中国科学院昆明动物研究所,昆明 650000  
谢丽分 中国科学院昆明动物研究所,昆明 650000  
张晓迪 中国科学院昆明动物研究所,昆明 650000  
邬继文 中国科学院昆明动物研究所,昆明 650000  
吕龙宝 1. 中国科学院昆明动物研究所,昆明 650000
2. 中国科学院昆明灵长类研究中心,昆明 650223 
lvlongbao@ mail. kiz.ac.cn 
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中文摘要:
      目的 应用高通量测序技术分析普通棉耳狨猴粪便菌群的结构与组成,为进一步开发利用新型实验动物奠定基础?方法 采集4 只成年雄性普通棉耳狨猴粪便,用细菌16S rRNA 通用引物扩增V3~ V4 区,采用Illumina MiSeq 测序平台对普通棉耳狨猴粪便微生物进行研究?结果 共测序获得315 511 条有效序列与596 个OTU?普通棉耳狨猴粪便中的细菌共鉴定出9 个门?14 个纲?26 个目?50 个科?82 个属和64 个种和226 个OTU?其中,1)优势门是拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),平均含量分别为54. 52% 和25. 39% 2)丰度最高的纲为拟杆菌纲( Bacteroidia) 和厌氧菌纲( Negativicutes), 平均含量为54. 5% 和17%?3) 乳杆菌目(Lactobacillales)和拟杆菌目(Bacteroidales)的丰度最高,为50. 01%和20. 52%?4)普雷沃菌科(Prevotellaceae)和双歧杆菌科(Bifidobacteriaceae)所占丰度最高,平均含量分别为43. 14%和11. 33%?5)双歧杆菌属(Bifidobacterium)和拟杆菌属(Bacteroides)的丰度较高,平均含量分别为11. 33% 11. 12%?6)有益菌群双歧杆菌属丰度较高,但在所检测样本中都含有?7)丰度最高的前10 个种,归类为7 个科?5 个纲?这10 个种占到33. 16%,其他53 种总和仅占到0. 74%,其他未鉴定出菌种,且相对丰度还较高,需要进一步研究?8) PICRUSt 功能预测分析:氨基酸转运等代谢功能和蛋白质翻译?折叠遗传信息处理功能丰度较高?结论 应用高通量测序技术,较全面的检测了普通棉耳狨猴粪便菌群,普通棉耳狨猴粪便细菌组成具有丰富的多样性,其中还有许多未被分类鉴定且相对丰度较高的细菌,需要进一步研究?
英文摘要:
      Objective To analyze the structure and composition of intestinal flora in the common cotton-earedmarmoset using the Illumina MiSeq sequencing platform. Methods Feces of the common cotton-eared marmoset werecollected and investigated for microbial diversity using the Illumina MiSeq sequencing platform. Results In total, 315511sequences and 596 OTUs were obtained after sequencing. According to the Shannon-Wiener curve, the sequencing datawere reliable for all bacteria in the samples. The bacteria in the common cotton-eared marmoset included 9 phyla, 14classes, 26 orders, 50 families, 82 genera, and 64 species. Among them, Firmicutes and Bacteroidetes had the highestabundance (54. 52% and 25. 39%, respectively). The dominant classes were Bacteroidia and Negativicutes at 54. 5% and17%, respectively. The abundance of Lactobacillales and Bacteroidales was higher at 50. 01% and 20. 52%, respectively.Dominant families were Prevotellaceae and Bifidobacteriaceae at 43. 14% and 11. 33% respectively. Lactobacillus andStreptococcus were dominant at 20. 03% and 19. 62%, respectively. The abundance of beneficial bacteria, such asBifidobacterium, was high, which were found in samples. The top 10 species with the highest abundance were classified intoseven families and five classes. These 10 species accounted for 33. 16%, and the other 53 species only accounted for0. 74%. Other species were not identified, and the abundance was relatively high, which require further study. PICRUStfunctional prediction showed an abundance of functional genes such as those involved in amino acid transport andmetabolism as well as genetic information processing. Conclusions The composition of fecal microbiota in common cottonearedmarmoset has rich diversity. Many bacteria that are relatively abundant remain unidentified, and further study is warranted.
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