Zebrafish has been widely used as an animal model for human disease research and drug screening.Bone disease models have been successfully established in zebrafish because of its highly conserved molecular mechanism of osteogenesis with mammals. Here, we introduce the processes and regulatory networks of zebrafish osteogenesis, and then summarize the classical method for zebrafish bone research and the research status of drug screening for bone diseases. This information will provide a reference for drug screening and basic research using zebrafish bone disease models.