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滕龙,洪芳,何建成.阴虚动风证帕金森病异动症模型大鼠的氧化应激反应及复方地黄方的干预作用[J].中国实验动物学报,2015,23(1):25~29.
阴虚动风证帕金森病异动症模型大鼠的氧化应激反应及复方地黄方的干预作用
Oxidative stress in the rat models of Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern, and the interventional effect of compound formula Rehmannia on the disease condition
投稿时间:2014-09-19  
DOI:10.3969/j.issn.1005-4847.2015.01.005
中文关键词:  帕金森病  阴虚动风证  异动症  复方地黄方  氧化应激  大鼠
英文关键词:Parkinson's disease  Dyskinesia  Yin deficiency stirring wind pattern  Rat
基金项目:国家自然科学基金(30973722);上海市科技支撑中药新药研究与开发项目(F50102);上海市卫生局中医药科研基金(2012J009A);基础医学院攀登计划。
作者单位E-mail
滕龙 上海中医药大学, 上海 201203  
洪芳 上海中医药大学, 上海 201203  
何建成 上海中医药大学, 上海 201203 hjc8163@163.com 
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中文摘要:
      目的 探讨阴虚动风证帕金森病异动症(LID)大鼠氧化应激反应及复方地黄方的干预作用。方法 采用6-羟基多巴胺(6-OHDA)偏侧损毁黑质制备帕金森病大鼠模型,进一步腹腔注射左旋多巴+苄丝肼(50 mg/kg左旋多巴和12.5 mg/kg苄丝肼)制备阴虚动风证帕金森病LID大鼠模型,并随机分为LID组、复方地黄方组,另取正常对照组、假手术组大鼠。每组6只。分别在4周、6周后进行神经行为学检测后,取纹状体,应用比色法分别测定各组大鼠纹状体内SOD、MDA、GSH、GSH-Px的含量。结果 阴虚动风证帕金森病LID模型组大鼠纹状体内SOD、GSH、GSH-Px的含量均明显的减少,MDA的含量呈增加的趋势;而复方地黄方组大鼠纹状体内SOD、GSH、GSH-Px的含量均呈现增加的趋势,且随治疗时间的延长增加趋势更明显,MDA的含量呈现减少的趋势,且随治疗时间的延长减少的趋势更明显。结论 复方地黄方较好地改善阴虚动风证LID大鼠的氧化应激反应,复方地黄方干预LID模型大鼠可能是通过清除自由基,减轻对细胞的损伤从而缓解帕金森病LID的症状。
英文摘要:
      Objective To explore the oxidative stress in Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern, and the interventional effect of compound formula rehmannia on the disease condition. Methods One hundred and twenty healthy male SD rats (body weight 180-200 g) were randomly divided into four groups: the control group, sham-operated group, model group, and Rehm group. The rat model with Parkinson's disease (PD) was established by injection of 6-hydroxyl dopamine to destroy the substantia nigra using the brain stereotaxic apparatus. Dyskinesia was induced in the PD rats by injection of levodopa into the brain. The dyskinesia PD model rats received intraperitoneal injection of levodopamine 50 mg/kg and benserazide 12.5 mg/kg for 2 weeks to induce Yin deficiency stirring wind pattern. The rats of Rehm group were given gastric gavage of compound formula rehmannia 2 mL/kg once a day for 4 weeks or 6 weeks. At the end of experiment (4 weeks and 6 weeks), neurobehavioral examination was performed, and the contents of SOD, MDA, GSH, and GSH-Px in the striatum tissue were assessed by colorimetry. Results In the LID group, the contents of SOD, GSH, GSH-Px in the striatum tissue were significantly lower than those in the normal control group and sham-operated group, and the malondialdehyde (MDA) content showed an increasing trend. In the compound rehmannia prescription group, the concentrations of SOD, GSH, GSH-Px in the striatum tissue showed an increasing trend, even more obvious along with the time course, and the malondialdehyde (MDA) content showed an decreasing trend, also even more obvious along with the time course. Conclusions The compound rehmannia prescription can improve the oxidative stress in rat models of Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern. The interventional effect of compound rehmannia prescription may be mediated by scavenging free radicals and reducing the damage to the cells, thus, to alleviate the symptoms of Parkinson's disease dyskinesia.
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