慢性心力衰竭大鼠肝脏组织代谢组学轮廓观察
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天津市卫生局攻关项目


Investigation of metabolic profiling of liver tissues in CHF rats
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    【摘要】目的 建立慢性心力衰竭大鼠肝脏组织代谢轮廓模型,从代谢角度探索慢性心衰肝脏代谢变化规律,探寻对心力衰竭分子机制及疾病诊疗具有价值的特征代谢物。方法 Wistar雄性大鼠20只,心衰组行腹主动脉缩窄手术,对照组行假手术,术后饲养24周。利用代谢组学研究平台对大鼠肝脏组织进行代谢轮廓分析,建立心衰大鼠肝脏组织正交偏最小二乘判别模型及主成分分析模型,结合 SPSS 19.0 软件的数据处理最终得到特征代谢物。结果 成功建立了主成分分析模型及正交偏最小二乘判别模型,同时从模型中筛选并鉴定了10种在心衰组与对照组具有差异的特征代谢物,包括溶血磷脂酰胆碱、 溶血磷脂酰乙醇胺,油酸,甘氨胆酸,硫酸脱氢表雄酮等物质。结论 构建的代谢轮廓模型很好地拟合慢性心衰大鼠肝脏的代谢紊乱状态,筛选出的特征代谢物可为该疾病的病理生理分子机制以及该疾病诊疗等提供参考和帮助。

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    Abstract: Objective A rat model of liver metabolism profile in chronic heart failure (CHF) was established for exploration of the dynamics of liver metabolism in CHF from the perspective of metabolism, in search for the characteristic metabolites significant for the molecular mechanism and management of CHF. Methods 20 male Wistar rats were assigned to the CHF group to receive aortic coarctation or to the control group to receive sham surgery, and were bred for 24 weeks following surgery. Metabolic profiling was performed on the rat liver tissues on a metabonomics research platform. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model and principal component analysis (PCA) model were established for liver tissues of CHF rats, and the characteristic metabolites were finally derived by data processing with SPSS 19.0 software. Results The PAC and OPLS-DA models were established successfully. 10 characteristic metabolites with differences between the CHF and control groups, e.g., lysophosphatidyl choline, lysophosphatidyl ethanolamine, oleic acid, glycocholic acid, and dehydroepiandrosterone sulfate, were screened and identified from the models. Conclusion The metabolic disorders in CHF rats were well fitted to the metabolic profile models established, and the characteristic metabolites identified could provide reference for the patho-physiological molecular mechanism and management, etc., of CHF.

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巫菲,李彤,张磊,董志欢,米睿.慢性心力衰竭大鼠肝脏组织代谢组学轮廓观察[J].中国比较医学杂志,,().

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  • 收稿日期:2016-03-16
  • 最后修改日期:2016-03-28
  • 录用日期:2016-06-15
  • 在线发布日期: 2016-11-28
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