Objective To evaluate the transplantation effect of erythroid progenitor cells in cloche^{-/ -} mutantzebrafish with congenital primary hematopoietic deficiency. Methods The embryos of zebrafish zTg (gata1:EGFP) werecollected and prepared into a single cell suspension. The gata1⁺ cells carrying green fluorescence were separated by flowcytometry and transplanted into the hearts of 42 hpf cloche^{-/ -} mutant zebrafish by microinjection. The expression of erythroidprogenitor cells in cloche^{-/ -} mutant zebrafish was examined by stereo fluorescence microscopy. Results gata1⁺ cells withgreen fluorescence were successfully selected. The proliferation of gata1⁺ cells with green fluorescence was observed at 2 hafter transplantation, and 16 h of continuous green fluorescence expression was observed. Conclusions Erythroidprogenitor cells have a potential to reconstruct hematopoiesis and provide an experimental basis for the further study of the functional identification of erythroid progenitor cells after transplantation.