Objective Echocardiographic techniques were used to study the changes in left ventricular (LV)function of cynomolgus monkeys with acute myocardial infarction (AMI), providing a reference for application of the cynomolgus monkey AMI model. Methods Cynomolgus monkeys were subjected to echocardiography before surgery, and the changes in LV morphology, LV hemodynamics, transmitral peak inflow velocity, and mitral annulus velocity were determined at 3 days and 1 month after modeling. Results Compared with that before modeling, the LVPWd of cynomolgus monkeys was increased significantly at 3 days after modeling ( P < 0. 05), and LVIDs were significantly increased at 1 month after modeling ( P < 0. 05). LVEF, SV, and LVFS indexes of the cynomolgus monkeys were significantly decreased at 3 days and 1 month after modeling ( P < 0. 05, P < 0. 01). CO was significantly decreased at 1 month after modeling ( P < 0. 05), whereas HR was significantly increased at 3 days after modeling ( P < 0. 05).Conclusions Echocardiographic techniques can be applied to LV cardiac function evaluation in the cynomolgus monkey AMI model. In particular, the indexes of LVEF, SV, and LVFS reflect the extent of AMI. The results of echocardiographic evaluation in the cynomolgus monkey AMI model are similar to those of human AMI.