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李文奇,冯 磊.性别对慢性交感应激导致心脏重塑的差异研究[J].中国比较医学杂志,2020,30(8):23~28,56.
性别对慢性交感应激导致心脏重塑的差异研究
Gender differences in cardiac remodeling induced by chronic sympathetic activation
投稿时间:2020-07-02  
DOI:10. 3969 / j.issn.1671-7856. 2020. 08. 004
中文关键词:  性别  慢性交感应激  心脏重塑
英文关键词:gender  chronic sympathetic activation  cardiac remodeling
基金项目:
作者单位E-mail
李文奇 江南大学药学院,江苏 无锡 214122 6171504015@ stu.jiangnan.edu.cn 
冯 磊 江南大学无锡医学院,江苏 无锡 214122 feng2008lei@ 163.com 
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中文摘要:
      目的 探究性别对慢性交感应激导致的心脏重塑是否存在差异。 方法 将小鼠随机分为四组:雄性对照组、雄性异丙基肾上腺素(ISO)组、雌性对照组、雌性 ISO 组。皮下注射异丙基肾上腺素 ISO 10 mg / (kg·d) 14 d,诱导小鼠心脏重塑模型。通过心脏重量与小鼠体重之比(HW/ BW)、心脏重量与胫骨长度之比(HW/ TL),评估心脏重塑大体指标;超声心动图评估小鼠心功能;HE 染色检测心肌细胞横截面积,超声心动图检测舒张末期室壁厚度,用于评估小鼠心脏肥大;天狼猩红染色用于评估心脏纤维化。 结果 雄性 ISO 组与对照组相比,心体比和 心胫比分别增加 9. 1%(P<0. 05)和 42. 8%(P<0. 001),雌性 ISO 组与对照组相比,心体比和心胫比分别增加 12. 9% (P<0. 05)和 9. 5%(P<0. 05),而与雌性 ISO 组相比,雄性 ISO 组心胫比增加了 19. 8%(P<0. 01);雄性 ISO 组与对照组相比,心肌细胞横截面积增加了 19. 1%(P<0. 0001),雌性 ISO 组与对照组相比,小鼠心肌细胞横截面积增加了 6. 9%(P<0. 05),而与雌性 ISO 组相比,雄性 ISO 组心肌细胞横截面积增加了 11. 6%(P<0. 01);与对照组相比,雄性和雌性 ISO 组 EF 值和 FS 值均无明显差异;雄性 ISO 组与对照组相比,小鼠心脏纤维化面积增加了 158%(P< 0. 0001),雌性 ISO 组与对照组相比,纤维化面积增加了 39. 7%(P<0. 05),与雌性 ISO 组相比,雄性 ISO 组心脏的纤维化面积增加了 119%(P<0. 0001)。 结论 性别对慢性交感应激导致的心脏重塑存在差异,与雌鼠相比,雄鼠更容易出现心脏肥大和心脏纤维化。
英文摘要:
      Objective To investigate whether there are gender differences in cardiac remodeling induced by chronic sympathetic activation. Methods Mice were randomly divided into four groups: male control, male isoprenaline (ISO), female control, and female ISO groups. A cardiac remodeling model was established by subcutaneous injection of ISO for 14 days. The ratio of heart weight to body weight (HW/ BW) and the ratio of heart weight to tibia length (HW/ TL) were used to assess cardiac remodeling; Cardiac function was assessed using echocardiography to determine diastolic left ventricular posterior wall thickness ( LVPW;d). Hematoxylin and eosin staining was used to determine myocyte cross- sectional area. Picric-sirius red staining was used to evaluate cardiac fibrosis. Results Compared with the control group, the HW/ BW and HW/ TL ratios were increased by 9. 1% (P<0. 05) and 42. 8% (P<0. 001), respectively, in the male ISO group and by 12. 9% and 9. 5% (all P<0. 05), respectively, in the female ISO group. The increase was significantly greater in the male ISO group (19. 8%, P< 0. 01) than in the female ISO group. Compared with the control group, the myocyte cross-sectional area in the male ISO group increased by 19. 1% (P<0. 0001), while that in the female ISO group increased by 6. 9% (P< 0. 05). The increase was significantly greater in the male ISO group ( P< 11. 6%) than in the female ISO group. There was no significant difference in cardiac function among groups. Compared with the control group, the cardiac fibrosis area of the male ISO group increased by 158% (P<0. 0001) and by 39. 7% (P<0. 05) in the female ISO group. Compared with the female ISO group, the fibrosis area of the male ISO group increased by 119% (P<0. 0001). Conclusions There are gender differences in cardiac remodeling induced by chronic sympathetic activation. Greater cardiac hypertrophy and cardiac fibrosis occur in male mice compared with female mice.
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