• 首页
  • 期刊介绍
  • 编委会
  • 投稿指南
  • 期刊订阅
  • 广告合作
  • 留言板
  • 联系我们
  • English
王晓宇,马富强,张晓宁,何 伟,宿杨帅,万红叶,胡 玲,景向红.不可预期应激刺激对大鼠消化道免疫和微生物菌群的影响[J].中国比较医学杂志,2020,30(8):1~9.
不可预期应激刺激对大鼠消化道免疫和微生物菌群的影响
Effects of chronic heterotypic intermittent stress stimulation on gastrointestinal immunity and microflora in rats
投稿时间:2019-10-09  
DOI:10. 3969 / j.issn.1671-7856. 2020. 08. 001
中文关键词:  不可预期应激  慢性刺激  皮质酮  胃黏膜  肠道微生物菌群
英文关键词:heterotypic intermittent stress  chronic stress  corticosterone  gastric mucosa  intestinal microflora
基金项目:
作者单位E-mail
王晓宇 中国中医科学院针灸研究所,北京 100700 xiaorain_wang@ hotmailcom 
马富强 洛阳市第一中医院,河南 洛阳 471000  
张晓宁 中国中医科学院针灸研究所,北京 100700  
何 伟 中国中医科学院针灸研究所,北京 100700  
宿杨帅 中国中医科学院针灸研究所,北京 100700  
万红叶 中国中医科学院针灸研究所,北京 100700  
胡 玲 中国中医科学院针灸研究所,北京 100700  
景向红 中国中医科学院针灸研究所,北京 100700 jxhtjb@ 263.net 
摘要点击次数: 533
全文下载次数: 143
中文摘要:
      目的 通过对 9 d 不可预期应激刺激干预后大鼠的观察,分析不可预期应激刺激对胃肠道免疫系统和微生物种群的影响,为慢性应激对胃肠道功能的研究提供参考。 方法 24 只 SD 大鼠随机分为空白组和慢性应 激模型组。模型组大鼠连续 9 d 接受不可预期应激刺激。造模完成后随机选择 5 只正常组和模型组大鼠,采集其粪便样本进行肠道微生物多样性分析。两组各 12 只大鼠中,随机选取 4 只进行灌流固定,取胃窦、结肠组织进行形态学观察;其余 8 只大鼠取血浆和肾上腺组织检测其中皮质酮的含量。 结果 (1)9 d 不可预期应激刺激后,模型组大鼠 9 d 体重增值与正常组大鼠相比明显减少(P= 0. 001),模型组大鼠肾上腺组织中皮质酮含量与正常组大鼠相比明显降低(P= 0. 006);模型组大鼠血浆中皮质酮含量与正常组大鼠相比增加(P= 0. 025)。 (2) 9 d 后模型 组大鼠的胃黏膜层上皮细胞排列较正常组稀疏,其厚度与正常组相比减少(P = 0. 034);模型组大鼠的胃黏膜固有 层中性粒细胞数量与正常组相比减少(P = 0. 016);模型组大鼠的结肠黏膜中中性粒细胞数量与正常组相比增多 (P= 0. 013)。 3)经过 9 d 慢性不可预期应激,Alpha 多样性分析显示,模型组大鼠粪便样本 OTU 中细菌种类与正常 组大鼠相比明显增加(P= 0. 001);其中模型组大鼠样本中脱硫弧菌科和螺杆菌科的占比为与正常组大鼠相比增加 (P= 0. 011,P= 0. 047);模型组大鼠样本中类杆菌科的占比与正常组大鼠相比明显减少,差异具有极显著的统计学意义(P= 0. 001)。 结论 9 d 不可预期应激刺激可以造成一个稳定的慢性应激大鼠模型,慢性应激所导致的免疫抑制与大鼠肠道微生物菌群失调伴随发生。
英文摘要:
      Objective To observe the effects of chronic heterotypic intermittent stress ( HIS ) on the gastrointestinal immune system, particularly in terms of (1) the morphology of the gastric and colonic mucosa, and (2) intestinal microbial diversity. Methods We randomly divided 24 SD rats into a control group and a model group. The rats in the model group received HIS for 9 days. After the 9 days of modeling, five rats in the control and model group were randomly selected, and fecal samples were collected for gut microbial diversity analysis. Four of the 12 rats from each of the two groups were randomly selected for perfusion and fixation, and tissues from the gastric antrum and colon were taken for morphological observation. Plasma and adrenal tissues were taken from the remaining eight rats for assessment of corticosterone content. Results (1) After 9 days of HIS, the rats in the model group had gained significantly less weight than those in the control group (P= 0. 001). Furthermore, the corticosterone content in adrenal tissue was significantly lower in the model group vs. the control group (P= 0. 006), and the corticosterone content in plasma was higher in the model group vs. the control group (P= 0. 025). (2) After HIS, the arrangement of gastric mucosal epithelial cells in the model group was thinner than that in the control group (P= 0. 034). Additionally, the number of neutrophils in the lamina propria of the gastric mucosa in the model group was lower than that in the control group (P= 0. 016). The number of neutrophils in the colonic mucosa in the model group was higher than that in the control group (P= 0. 013). (3) After 9 days of HIS, Alpha diversity analysis showed that the number of operational taxonomic units in the fecal samples from the model group was significantly higher than that in the control group (P= 0. 001). The proportion of desulfovibrionaceae and helicobacteraceae in the model group was higher than that in the control group (P= 0. 011, P= 0. 047). Furthermore, the proportion of bacteroidaceae in the model group was significantly lower than that in the control group ( P= 0. 001). Conclusions Nine days of HIS induced a stable rat model of chronic stress in which chronic stress-induced immunosuppression occurred concomitantly with dysregulation of the gut microbial flora.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 3888418 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会 中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码