高脂血症食蟹猴模型的建立及初步分析
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(1.广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州 510260; 2.华南农业大学实验动物中心,广州 510642)

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R-33

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Establishment and preliminary analysis of a hyperlipidemic cynomolgus monkey model
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(1. Guangdong Key Laboratory of Animal Conservation and Resource Utilization; Guangdong Public Laboratory of Wild Animal Conservation and Utilization; Guangdong Institute of Applied Biological Resources, Guangzhou 510260, China.2. Laboratory Animal Center, South-China Agricultural University, Guangzhou 510642)

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    摘要:

    目的 建立与人类疾病特征相似的高血脂食蟹猴模型,为降脂药(特别是生物类降脂药)的研发提供理想的动物模型?方法 将优化?定型的半流质高糖高脂膳食乳剂,每周6 d 经鼻饲灌喂给药处理组雄性食蟹猴,定期监测猴体重(BW)?体重质量指数(BMI)?血清总胆固醇(TC)?甘油三酯(TG)?高密度脂蛋白胆固醇(HDLC)?低密度脂蛋白胆固醇(LDL-C)等指标,对其血脂指标的变化趋势进行分析?结果 与相应的对照组相比,至造模第3 个月,处理组6 只中老年雄性食蟹猴和5 只成年雄性食蟹猴全部造模成功,至造模第21 个月所有处理组雄性食蟹猴依然维持在较高血脂水平?结论 连续3 个月定量灌喂半流质高糖高脂膳食乳剂可成功制备高脂血症食蟹猴模型,该模型具有高胆固醇血症和高低密度脂蛋白胆固醇血症的特征,可用于高脂血症发病机理研究及药物临床前药效评价研究?

    Abstract:

    Objective To establish a hyperlipidemic cynomolgus monkey model with human diseasecharacteristics, and to provide an ideal animal model for the development of lipid-lowering drugs, especially lipid-loweringbiopharmaceuticals. Methods The male cynomolgus monkeys in different treatment groups were gavaged with a formulatedsemi-liquid high fat/ high sugar dietary emulsion 6 days per week. Body weight (BW), body mass index (BMI), serumtotal cholesterol ( TC), triglyceride ( TG), high density lipoprotein cholesterol ( HDL-C), low density lipoproteincholesterol (LDL-C), and other indexes were monitored regularly, and changes in the blood lipid profiles were analyzed.Results Compared with the corresponding control group, by the third month of modeling, all six middle-aged and old malecynomolgus monkeys and five adult male cynomolgus monkeys in the treatment group were successfully modeled. By the 21stmonth, all male cynomolgus monkeys in the treatment group still maintained a relatively high level of blood lipid.Conclusions A hyperlipidemic cynomolgus monkey model is successfully created through quantitative gavage using a highsugar and high fat dietary emulsion for 3 months. The model demonstrates the characteristics of hypercholesterolemia andhigh-density lipoprotein cholesterolemia, and it can be used to study the pathogenesis of hyperlipidemia and for pre-clinical drug evaluations of pharmacodynamics.

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唐明甜,肖百全,徐世千,李比海,刘忠华,饶军华.高脂血症食蟹猴模型的建立及初步分析[J].中国比较医学杂志,2019,29(7):61~66.

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  • 收稿日期:2019-01-29
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  • 在线发布日期: 2019-08-01
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