高蛋白高钙饮食合并限制饮水建立鹌鹑痛风模型初探
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(北京中医药大学中药学院,北京 100029)

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R-33

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Preliminary study on the establishment of a quail model of gout induced by high-protein and high-calcium diet combined with restricted drinking water
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(School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China)

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    摘要:

    目的 通过模拟临床高蛋白高钙饮食诱导迪法克鹌鹑痛风,初步探讨建立鹌鹑痛风模型的方法,为痛风发病机制研究及防治药物筛选提供依据?方法 选用36 只30 日龄雄性健康迪法克鹌鹑,以体重和血尿酸水平随机分为3 组,即对照组?模型I 组和模型II 组,每组12 只?对照组鹌鹑饲喂基础日粮,模型组饲喂高蛋白高钙日粮(日粮粗蛋白含量29. 5%,钙含量7. 8%),对照组?模型I 组自由饮水,模型II 组每日限制饮水4 h,实验持续50 d?检测鹌鹑血清?粪尿尿酸水平,同时检测肾功能并进行组织学病理切片观察?结果 高蛋白高钙日粮可使鹌鹑血清尿酸水平显著升高;粪尿尿酸水平显著降低;血清肌酐?尿素氮水平升高;导致鹌鹑肾尿酸盐沉积明显?高蛋白高钙日粮合并限制饮水可加剧血尿酸水平升高?肾尿酸盐沉积,使部分鹌鹑出现关节肿大?变形等痛风症状?结论 饲喂高蛋白高钙日粮并限制饮水量可以建立痛风鹌鹑模型,其发病过程与临床痛风病因?发病机制及临床表现更为符合,为痛风病的基础及药物开发研究提供参考?

    Abstract:

    Objective To establish a quail model of gout by simulating a clinical high-protein and high-calciumdiet, and to provide a basis for studying gout pathogenesis and drug screening for its prevention and treatment. Methods Tirty-six 30-day-old healthy male quails were randomly and equally divided into three groups according to body weight andblood uric acid level, namely, the control group, model I group, and model II group. The control group was fed the basaldiet, and the model groups were fed a high-protein high-calcium diet (dietary crude protein content of 29. 5% and calciumcontent of 7. 8%). The control group and the model I group were provided with drinking water ad libitum, while the modelII group was given drinking water restricted to 4 h daily. The experiment lasted for 50 days. The serum and fecal uric acidlevels were measured, renal function was determined, and histopathological examination was performed. Results Thehigh-protein and high-calcium diet significantly increased serum uric acid levels, significantly reduced fecal uric acidlevels, and did not change the serum creatinine and urea nitrogen levels. The high-protein and high-calcium diet combinedwith restricted drinking water aggravated the increase of blood uric acid level and renal urate deposition, causing goutchanges such as joint swelling and deformation. Conclusions A gout model can be established by feeding on a high-proteinand high-calcium diet and limiting the amount of drinking water. The pathogenesis of gout in this model is more consistentwith the etiology, pathogenesis, and clinical manifestations of gout in clinics, providing a reference for the research on gout pathogenesis and drug development.

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王海鸽,林志健,张冰,李高玺,王笑.高蛋白高钙饮食合并限制饮水建立鹌鹑痛风模型初探[J].中国比较医学杂志,2019,29(4):15~22.

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  • 收稿日期:2018-08-08
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  • 在线发布日期: 2019-05-10
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