汉黄芩素体外对胃癌SGC-7901 细胞增殖、迁移和侵袭的实验性研究
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(信阳职业技术学院检验技术学院,河南信阳 464000)

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R-33

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Inhibitory effects of wogobin on proliferation, migration, and invasion of gastric cancer SGC-7901 cells in vitro
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(Inspection Technology College of Xinyang Vocational and Technical College,Xinyang 464000,China)

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    摘要:

    目的 探讨汉黄芩素对胃癌细胞增殖?迁移和侵袭的影响以及可能分子机制?方法 不同浓度(20?50?100 μmol/ L) 汉黄芩素分别作用于人胃癌SGC7901 细胞24?48?72 h,空白对照不进行任何处理?采用MTT 法检测细胞增殖能力;采用划痕实验观察细胞迁移能力;采用Transwell 法观察细胞侵袭能力;采用流式细胞仪检测细胞周期与凋亡情况;RT-qPCR 和Western blot 分别对细胞基质金属蛋白酶2?9(MMP2?MMP9)?细胞间黏附分子-1(ICAM-1)以及金属蛋白酶抑制剂2(TIMP2)的mRNA 和蛋白表达水平进行检测?结果 MTT 实验结果显示,不同浓度的汉黄芩素抑制人胃癌SGC7901 细胞呈浓度和时间依赖性;作用48 h 后,汉黄芩素s 期比例上升,诱导细胞凋亡( P <0. 05);Transwell 实验结果显示,汉黄芩素呈浓度依赖性抑制人胃癌SGC7901 细胞侵袭;RTqPCR和Western blot 检测结果显示,汉黄芩素能够抑制SGC7901 细胞中ICAM-1?MMP9?MMP2 的mRNA 和蛋白表达水平,但上调TIMP2 mRNA 和蛋白表达( P <0. 05)?结论 汉黄芩素能够抑制人胃癌SGC7901 细胞增殖?迁移和侵袭,阻滞细胞在S 周期,诱导其凋亡?

    Abstract:

    Objective To investigate the effects of wogobin on the proliferation, migration, and invasion of gastric cancer cells and their molecular mechanisms. Methods Different concentrations (20, 50, and 100 mol/ L) of wogobin were used to treat the hepatoma cell line SGC7901 cells for 24, 48, and 72 h. In the blank control, no treatment was performed. MTT assay was used to determine the cell proliferation. The migration ability of cells was observed by a scratch test. The Transwell method was used to observe cell invasiveness. Cell cycle and apoptosis were determined by flow cytometry. The mRNA and protein expression levels of cellular MMP2, MMP9, ICAM-1, and TIMP2 were determined by RT-qPCR and western blotting, respectively. Results MTT assay showed that the different concentrations of wogobin inhibited the hepatoma cell line SGC7901 in a concentration- and time-dependent manner. After 48 h, wogobin arrested the cell cycle at S-phase and induced the apoptosis in gastric cancer cells ( P <0. 05). Transwell assay showed that wogobin inhibited the invasion of the SGC7901 cells in a concentration-dependent manner. The RT-qPCR and western blotting analyses showed that wogobin inhibited the mRNA and protein expression of MMP9, MMP2, and ICAM-1 in SGC7901cells, but upregulated TIMP2 ( P <0. 05). Conclusions Wogobin inhibites the proliferation, migration, and invasion of the hepatoma cell line SGC7901 cells and induces its apoptosis.

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张永海.汉黄芩素体外对胃癌SGC-7901 细胞增殖、迁移和侵袭的实验性研究[J].中国比较医学杂志,2019,29(2):89~95.

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  • 收稿日期:2018-08-06
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  • 在线发布日期: 2019-03-14
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