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唐涛,马小翠,姚冰,陈一,朴浩哲.大鼠脊髓挫伤模型对比及行为学评价[J].中国比较医学杂志,2019,29(2):84~88.
大鼠脊髓挫伤模型对比及行为学评价
Comparison and behavioral evaluation of rat models of spinal cord contusion
投稿时间:2018-08-04  
DOI:10.3969/j. issn. 1671 -7856. 2019. 02. 014
中文关键词:  脊髓损伤  模型  动物  行为学
英文关键词:spinal cord injury  model  animal  behavioristics
基金项目:
作者单位E-mail
唐涛 神经外科,中国医科大学肿瘤医院,辽宁省肿瘤医院,沈阳 110042 taotang006@ 163.com 
马小翠 麻醉科,中国医科大学肿瘤医院,辽宁省肿瘤医院,沈阳 110042  
姚冰 神经外科,中国医科大学肿瘤医院,辽宁省肿瘤医院,沈阳 110042  
陈一 神经外科,中国医科大学肿瘤医院,辽宁省肿瘤医院,沈阳 110042  
朴浩哲 神经外科,中国医科大学肿瘤医院,辽宁省肿瘤医院,沈阳 110042 pzpy@ 163.com 
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中文摘要:
      目的 建立不同程度MASCIS 和IH 大鼠脊髓损伤模型,研究损伤程度?造模方式与行为?解剖结果之间的关系?方法 将80 只雌性SD 大鼠随机分为4 组,每组20 只?分别制作NYU 12. 5 mm?NYU 25 mm?IH 150kdyn 和IH 200 kdyn 模型,对模型制作成功率?BBB 评分?Grid Walking 评价?热痛以及损伤中心残余组织量进行评价?结果 两种造模方法成功率相似;所有动物损伤后0~3 dBBB 评分均降至0 分左右,随时间增加,BBB 评分均逐渐恢复,4~6 周达到平台期,NYU 25 mm 组评分最低,IH 200 组评分居中,IH 150 组合NYU 12. 5 mm 组评分相似且最高;Grid Walking 坠落率随损伤程度增高而增加;Hargreaves 结果受运动功能影响较大;运动障碍与损伤中心层面组织残余量成反比?结论 MASCIS 和IH 模型均能满足脊髓损伤病理生理机制研究要求,但应根据实验目的选取相适应的造模参数?
英文摘要:
      Objective To establish animal models of spinal cord injury of different degrees of severity, namely,MASCIS and IH, and to study the relationship among the degree of damage, modeling mode, behavior, and anatomical results. Methods Eighty female SD rats were randomly and equally divided into four groups: NYU 12. 5 mm, NYU 25mm, IH 150 kdyn force, and IH 200 kdyn force models. Their rates of successful model establishment, BBB score, grid walking evaluation, Hargreaves test, and total cross-sectional area of the spinal cord at the injury epicenter were evaluated.Results The success rates in the two modeling methods were similar. The BBB scores of all animals were reduced to 0points at 0 to 3 days after spinal cord injury. As time passed, the BBB scores gradually recovered and reached a plateau at 4to 6 weeks. The NYU 25 mm group scored the lowest, the IH 200 group had an intermediate score, and the IH150 and NYU 12. 5 mm groups scored similarly and highest. The grid walking score increased with increasing injury severity. The Hargreaves test results were more affected by motor function. Dyskinesia was inversely proportional to the residual amount of tissue at the center of the injury. Conclusions Both MASCIS and IH models are appropriate for research on the pathophysiological mechanisms of spinal cord injury, but appropriate modeling parameters should be selected according to the purpose of the experiment.
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