诺如病毒感染树鼩小动物模型的初步探索与思考
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(1.南方医科大学中医药学院,广州 510515;2.南方医科大学公共卫生学院流行病学系,广州 510515)

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R-33

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Preliminary exploration and thinking of a small animal model of human norovirus infection in tree shrews
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(1. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.2. Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou 510515)

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    摘要:

    目的 探索通过灌胃攻毒的方法建立树鼩诺如病毒(Human Noroviruses, HuNoVs)感染动物模型的可行性?方法 分别灌胃两组6 月龄树鼩攻毒GII.4 型HuNoVs 2010 和Sydney 株粪便悬液,对照组灌同等体积的PBS(每组5 只),攻毒后连续7 d 观察体征和腹泻症状,每隔24 h 采集粪便应用RT-PCR 法检测HuNoVs RNA,处死树鼩后采集小肠组织,HE 染色观察病理变化并应用免疫组化法检测病毒抗原,并对树鼩唾液进行人类组织血型抗原(histoblood group antigen,HBGAs)鉴定分析?结果 树鼩经HuNoVs 灌胃攻毒后未出现明显感染症状和腹泻,粪便标本中未检测到HuNoVs RNA,小肠组织中未检测到HuNoVs 抗原,病理变化不明显,树鼩唾液中HBGAs 抗原为阴性?结论 树鼩对HuNoVs 不易感原因可能与其不分泌表达HBGAs 和普通级饲养繁殖条件等有关,HuNoVs 感染树鼩小动物模型仍有待进一步探索?

    Abstract:

    Objective To explore the feasibility of establishing a tree shrew model of Human Noroviruses (HuNoVs) infection by inoculation with HuNoVs. Methods Three groups of 6-month-old tree shrews (five in each group) were treated with GII.4 type HuNoVs 2010, Sydney strains or an equal dose of PBS (control). Physical signs and diarrhea were observed for 7 days after HuNoVs challenge. HuNoVs RNA was detected by RT-PCR using stool samples collected every 24 h, and then the tree shrews were sacrificed to collect the small intestinal tissues. HE staining was used to observe pathological changes. The presence of HuNoVs antigen was tested by immunohistochemistry (IHC), and the saliva from tree shrews was analyzed for histoblood group antigen(HBGAs)by ELISA. Results No obvious infection symptoms or diarrhea were observed in the tree shrews after HuNoVs challenge. No HuNoVs RNA was detected in stool specimens, and no HuNoVs antigen was detected in the small intestine. No pathological changes were observed. HBGAs antigen was negative in the saliva of tree shrews. Conclusions Tree shrews are not susceptible to HuNoVs possibly because of the lack of HBGAs expression and common breeding conditions. The small animal model of tree shrews infected with HuNoVs remains to be further explored.

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郭伦爱,戴迎春,陈俊锐,段文韬,张绪富.诺如病毒感染树鼩小动物模型的初步探索与思考[J].中国比较医学杂志,2019,29(2):14~18.

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  • 收稿日期:2018-07-30
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  • 在线发布日期: 2019-03-14
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