姜黄素预处理对干热环境下不同程度中暑大鼠肾病理损伤及氧化应激的影响
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(1. 新疆军区总医院新疆特殊环境医学重点实验室,乌鲁木齐 830000; 2. 解放军69240 部队医院,乌鲁木齐 830000)

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R-33

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Effect of curcumin pretreatment on renal pathological changes and the level of oxidative stress in rats with various degrees of heat stroke in a dry heat environment
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(1. Key Laboratory of the Special Environmental Medicine of Xinjiang, General Hospital of Xinjiang Military Command, Urumqi 830000, China. 2. The No. 69240 Army Hospital, Urumqi 830000)

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    摘要:

    目的 观察姜黄素预处理对不同程度中暑大鼠肾病理变化以及肾组织氧化应激9 水平的影响?方法 雄性健康SD 大鼠80 只,随机分为2 组(n =40):盐水对照组以及姜黄素预处理组?每组再分为4 个亚组(n =10):干热0 min 组(即常温组),干热50 min 组(轻度中暑组),干热100 min 组(中度中暑组),干热150 min 组(重度中暑组)?盐水组大鼠给予0. 9%生理盐水灌胃,姜黄素组大鼠给予剂量为200 mg/ kg 姜黄素灌胃;各组大鼠均连续灌胃7 d?0 min 组置于常温环境中,其余组放置在西北特殊环境人工实验舱内,设置干热环境温度:(41 ±0. 5)℃,湿度(10 ±1)%,并在相应时间点麻醉大鼠,取血液以及肾组织?检测血液中肌酐及尿素氮含量;肾组织制成匀浆进行超氧化物歧化酶(superoxide dismutase)?过氧化氢酶(catalase)以及丙二醛(malondialdehyde)活性的检测;将肾组织制片,HE 染色,观察病理变化?结果 病理学检测发现,肾组织病理变化及评分随着干热环境时间的延长而加重,在同一时间点姜黄素预处理组大鼠肾病理损伤评分明显低于盐水组( P < 0. 05, P < 0. 01);血清肌酐?尿素以及肾组织中MDA 含量在三个中暑阶段中,姜黄素组均明显低于盐水组( P < 0. 05);肾组织中抗氧化酶SOD?CAT 的活性姜黄素组均明显高于盐水组( P < 0. 05),经相关性分析提示,MDA 与病理损伤评分密切相关(r =0. 75, P < 0. 01)?结论 姜黄素预处理对干热环境下不同程度中暑大鼠肾损伤均具有保护作用,其可能部分通过抗氧化应激反应来实现?

    Abstract:

    Objective To observe the effects of curcumin pretreatment on renal pathological changes and the level of oxidative stress in rats with various degrees of heat stroke. Methods Eighty male healthy SD rats were randomly divided into two groups (n =40): saline control and curcumin pretreatment groups. Each group was divided into four subgroups (n=10): 0 min (normal temperature), 50 min, 100 min, and 150 min groups in dry heat environment. Rats of the saline group were administered 0. 9% normal saline, and the curcumin group was administered 200 mg/ kg curcumin. The rats were treated continuously for 7 days. Rats of the 0 min group were subjected to room temperature, while the other groups were transferred to a climate cabin (The Simulated Climate Cabin for Special Environment of Northwest of China) under the conditions of 40. 5 ±0. 5°C and 10 ±1% relative humidity to establish the rat heat stroke model by a dry heat environment.The rats were anesthetized at the corresponding time points, and blood and kidney tissue were collected. The contents of creatinine and urea nitrogen in serum and superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA)activities in homogenates of kidney tissue were detected. Renal tissue was stained with HE, and pathological changes were observed. Results Pathological changes of renal tissue were aggravated by prolonged exposure to the dry heat environment. Pathological renal injury scores were higher in the saline group compared with curcumin pretreatment groups at the same time points ( P <0. 05, P <0. 01). The contents of serum creatinine, urea, and MDA were decreased in the curcumin groups compared with the saline group at the three heatstroke periods ( P <0. 05). The activities of renal tissue antioxidant enzymes SOD and CAT were increased in the curcumin group compared with the saline group ( P < 0. 05). Correlation analysis indicated that renal injury scores were correlated with MDA (r = 0. 75, P < 0. 01). Conclusions Curcumin pretreatment has a protective effect on rat renal injury induced by various degrees of heat stroke in a dry heat environment, and its mechanism may be mediated partly by antioxidant effects.

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李建瑛,肇寅辉,康燕,张东辉,许琴,董翔,李佳佳,是文辉,马娜,沈才福,刘江伟.姜黄素预处理对干热环境下不同程度中暑大鼠肾病理损伤及氧化应激的影响[J].中国比较医学杂志,2018,28(11):48~52.

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  • 收稿日期:2018-04-04
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  • 在线发布日期: 2018-12-18
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