4T1 炎性乳腺癌细胞接种联合皮质酮注射诱导乳腺癌并发抑郁症小鼠模型的研究
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(1. 湖南中医药大学第一附属医院中心实验室,长沙 410007; 2. 湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地,长沙 410208; 3. 湖南省中医药研究院,长沙 410006)

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R-33

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4T1 inflammatory breast cancer cell inoculation combined with corticosterone injection to establish a mouse model of breast cancer complicated with depression
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(1. Central Laboratory, the First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha 410007, China. 2. National Key Laboratory of Chinese Materia Medica Powder and Innovation Drugs Established by Province and Ministry, Hunan University of Chinese Medicine, Changsha 410208. 3. The Hunan Provincial Institute of Traditional Chinese Medicine, Changsha 410006)

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    摘要:

    目的 研究4T1 炎性乳腺癌细胞接种联合皮质酮注射所致BABL/ c 小鼠病理行为和核心标志物的变化?方法 采用4T1 炎性乳腺癌细胞接种的方法建立乳腺癌小鼠模型,并随机分为2 组,乳腺癌组和乳腺癌皮质酮组;另将未接种乳腺癌细胞的小鼠也随机分为2 组,正常对照组和抑郁症组?随后给予抑郁症组和乳腺癌皮质酮组21 d 皮质酮注射(30 mg/ kg)?实验结束后,采用糖水消耗实验?旷场实验?新奇摄食实验观察各组小鼠的抑郁样行为?小鼠断头处死后,测量各组小鼠乳腺肿瘤瘤重和瘤体积,采用ELISA 法检测小鼠肿瘤标志物肿瘤抗原CA153?糖类抗原CA125?癌胚抗原(CEA)?5-羟色胺(5-HT)?多巴胺(DA)?去甲肾上腺素(NE)的含量?结果 乳腺癌皮质酮组小鼠瘤重和瘤体积明显增大,血浆CA153?CA125 和CEA 水平显著升高,糖水偏嗜度明显降低,对环境的好奇探索欲望减弱,体内5-HT?DA?NE 含量显著降低?结论 乳腺癌小鼠在接受慢性皮质酮注射后,可表现出明显的抑郁样行为?神经递质和乳腺癌标志物以及肿瘤瘤体变化,可为乳腺癌并发抑郁症动物实验提供模型参考?

    Abstract:

    Objective To investigate changes of the pathological behaviors and core markers in BALB/ c mice treated by 4T1 inflammatory breast cancer cells inoculation and corticosterone injection. Methods Breast cancer mouse mod els were established by inoculation of 4T1 inflammatory breast cancer cells and randomly divided into two groups: breast cancer and breast cancer with corticosterone groups. Healthy mice were randomly divided into two groups: normal control and depression groups. Subsequently, corticosterone injections (30 mg/ kg, 21 days) were administered to the depression and breast cancer with corticosterone groups. At the end of the experiment, depression-like behaviors of mice in each group were assessed by sugared water consumption, open field, and novel feeding tests. Then, all the mice were sacrificed by decapitation, and the tumor weight and volume in each mouse were measured. The levels of tumor antigens carbohydrate antigen (CA) 153, CA125, carcinoembryonic antigen (CEA), and 5-hydroxytryptamine (5-HT), dopamine(DA), norepinephrine (NE) were detected by ELISA. Results In the breast cancer with corticosterone group, the tumor weight and volume were increased significantly, and the levels of CA153, CA125, and CEA were increased significantly. Furthermore, the degree of sugar-water bias was reduced significantly, and the desire to explore the environment was weakened in the breast cancer mouse with corticosterone group. The concentrations of 5-HT, DA, and NE were reduced significantly. Conclusions After corticosterone treatment, breast cancer-bearing mice exhibit obvious depression-like behaviors. Neurotransmitters, breast cancer markers, and tumors are also changed. Based on the above indicators, this method of 4T1 inflammatory breast cancer cell inoculation combined with corticosterone injection can generate mouse model of breast cancer complicated with depression, which provides a reference for animal depression experiments.

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杨蕙,孟盼,杨琴,韩远山,凌佳,杜青,赵洪庆,王宇红.4T1 炎性乳腺癌细胞接种联合皮质酮注射诱导乳腺癌并发抑郁症小鼠模型的研究[J].中国比较医学杂志,2018,28(11):21~26.

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  • 收稿日期:2018-04-04
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  • 在线发布日期: 2018-12-18
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